{"refrec":{"BRefID":347382,"RR":"West, K.M.; Travers, M.J.; Stat, M.; Harvey, E.S.; Richards, Z.T.; Dibattista, J.D.; Newman, S.J.; Harry, A.; Skepper, C.L.; Heydenrych, M.; Bunce, M. (2021). Large‐scale eDNA metabarcoding survey reveals marine biogeographic break and transitions over tropical north‐western Australia. Diversity Distrib. 27(10): 1942-1957. https://dx.doi.org/10.1111/ddi.13228","BEntID":344074,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". Diversity Distrib. 27(10): 1942-1957. https://dx.doi.org/10.1111/ddi.13228","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"West, K.M.; Travers, M.J.; Stat, M.; Harvey, E.S.; Richards, Z.T.; Dibattista, J.D.; Newman, S.J.; Harry, A.; Skepper, C.L.; Heydenrych, M.; Bunce, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"West, K.M. et al.","Englishabstract":"

Aim

Environmental DNA (eDNA) metabarcoding has demonstrated its applicability as a highly sensitive biomonitoring tool across small spatial and temporal scales in marine ecosystems. However, it has rarely been tested across large spatial scales or biogeographical barriers. Here, we scale up marine eDNA metabarcoding, test its ability to detect a major marine biogeographic break and evaluate its use as a regional biomonitoring tool in Australia.

Location

North-western Australia (NWA).

Methods

We applied metabarcoding assays targeting the mitochondrial 16S rRNA and CO1 genes to 284 surface seawater eDNA samples collected from 71 mid-shelf, inshore, coastal and nearshore estuarine sites over 700 km of the NWA coastline.

Results

Metabarcoding detected a wide range of bony fish (404 taxa), elasmobranchs (44) and aquatic reptiles (5). We detected bioregional and depth differentiation within inshore bony fish communities. These findings support the presence of a marine biogeographic break, which is purported to occur in the vicinity of Cape Leveque, demarcating the border between the Kimberley and Canning bioregions. Inshore bony fish and elasmobranch communities, as well as coastal bony fish assemblages, were additionally found to differ between the South and North Kimberley regions suggesting previously unrecognized subregional differentiation amongst these taxa. The overall compositional data have been used to update distribution information for a number of endangered, elusive and data-deficient taxa, including sawfish (family: Pristidae), northern river shark (Glyphis garricki) and wedgefish (genus: Rhynchobatus).

Main conclusions

eDNA metabarcoding demonstrated a high level of sensitivity that was able to discern fine-scale patterns across the large-scale, remote and oceanographically complex region of North-western Australia. Importantly, this study highlights the potential of integrating broad-scale eDNA metabarcoding alongside other baseline surveys and long-term monitoring approaches, which are crucial for the sustainable management and conservation of marine biodiversity in this unique marine region.

","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Large‐scale eDNA metabarcoding survey reveals marine biogeographic break and transitions over tropical north‐western Australia","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-02 01:33:20.623832","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"biogeographic; biomonitoring; elasmobranch; environmental DNA; Kimberley; large-scale; marinebiodiversity; marine reptile; teleost; threatened species","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-11-18","DateCreate":"2021-11-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000610910100001","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/ddi.13228"},"refs":null,"anarec":{"AnaID":347382,"PubliDate":2021,"Pagination":"1942-1957","XtraPublOfAnaID":null,"ISBN":null,"Volume":"27","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":99812,"SerRR":"Diversity and Distributions. Blackwell: Oxford. ISSN 1366-9516; e-ISSN 1472-4642","StandardTitleSer":"Diversity and Distributions","ISSN":"1366-9516","AbbrevSer":"Diversity Distrib.","StandardTitleMon":null,"StartPage":1942,"Pages":16,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"West","Firstname":"Katrina","Initials":"K.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":417544,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Travers","Firstname":"Michael","Initials":"M.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":417550,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Stat","Firstname":"Michael","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":174107,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Harvey","Firstname":"Euan","Initials":"E.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":134679,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Richards","Firstname":"Zoe","Initials":"Z.T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":79769,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dibattista","Firstname":"Joseph","Initials":"J.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":173801,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Newman","Firstname":"Stephen","Initials":"S.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":5395,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Harry","Firstname":"Alastair","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":470228,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Skepper","Firstname":"Craig","Initials":"C.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":417547,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Heydenrych","Firstname":"Matthew","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":470230,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bunce","Firstname":"Michael","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":344074,"AutID":273272,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":369267,"LibID":36,"BRefID":347382,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"369267","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Elasmobranchii"},{"TaxTerm":"Reptilia"},{"TaxTerm":"Teleostei"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":344074,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":344074,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/ddi.13228","externalID":"10.1111/ddi.13228","URLTypeCode":"DOI","URLID":97636,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Elasmobranchii","AphiaID":10193,"TaxtID":20609},{"TaxTerm":"Reptilia [reptiles]","AphiaID":1838,"TaxtID":19286},{"TaxTerm":"Teleostei","AphiaID":293496,"TaxtID":23936}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Elasmobranchii","DutchTerm":"Kraakbeenvissen","AphiaID":10193,"TaxtID":20609},{"TaxTerm":"Reptilia","DutchTerm":"reptielen","AphiaID":1838,"TaxtID":19286},{"TaxTerm":"Teleostei","DutchTerm":null,"AphiaID":293496,"TaxtID":23936}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"VLIZ2000\\liesjes","newSesDate":{"date":"2021-11-18 09:52:00.370000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"VLIZ2000\\liesjes","updSesDate":{"date":"2021-11-18 09:52:00.370000","timezone_type":3,"timezone":"Europe/Brussels"}}}