Document of bibliographic reference 347574

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Development and validation of a multi-locus PCR-HRM method for species identification in Mytilus genus with food authenticity purposes

Abstract

DNA-based methods using informative markers such as single nucleotide polymorphism (SNPs) are suitable for reliable species identification (SI) needed to enforce compliance with seafood labelling regulations (EU No.1379/2013). We developed a panel of 10 highly informative SNPs to be genotyped by PCR-High resolution melting (HRM) for SI in the Mytilus genus through in silico and in vitro stages. Its fitness for purpose and concordance were assessed by an internal validation process and by the transference to a second laboratory. The method was applicable to identify M. chilensis, M. edulis, M. galloprovincialis and M. trossulus mussels, fresh, frozen and canned with brine, oil and scallop sauce, but not in preserves containing acetic acid (wine vinegar) and tomato sauce. False-positive and negative rates were zero. Sensitivity, expressed as limit of detection (LOD), ranged between 5 and 8 ng/μL. The method was robust against small variations in DNA quality, annealing time and temperature, primer concentration, reaction volume and HRM kit. Reference materials and 220 samples were tested in an inter-laboratory assay obtaining an “almost perfect agreement” (κ = 0.925, p < 0.001). In conclusion, the method was suitable for the intended use and to be applied in the seafood industry.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000689327100001

Bibliographic citation

Quintrel, M.; Jilberto, F.; Sepúlveda, M.; Marín, M.E.; Véliz, D.; Araneda, C.; Larraín, M.A. (2021). Development and validation of a multi-locus PCR-HRM method for species identification in Mytilus genus with food authenticity purposes. Foods 10(8): 1684. https://dx.doi.org/10.3390/foods10081684

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true