Document of bibliographic reference 347575

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: digestion method and viability studies

Abstract

Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000675152200001

Bibliographic citation

Duflot, M.; Midelet, G.; Bourgau, O.; Buchmann, K.; Gay, M. (2021). Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: digestion method and viability studies. J. Fish Dis. 44(11): 1777-1784. https://dx.doi.org/10.1111/jfd.13495

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true