{"refrec":{"BRefID":349193,"RR":"<b>Fernandes, H.; Salgado, J.M.; Martins, N.; Peres, H.; Oliva-Teles, A.; Belo, I.</b> (2019). Sequential bioprocessing of <i>Ulva rigida</i> to produce lignocellulolytic enzymes and to improve its nutritional value as aquaculture feed. <i>Bioresour. Technol. 281</i>: 277-285. <a href=\"https://dx.doi.org/10.1016/j.biortech.2019.02.068\" target=\"_blank\">https://dx.doi.org/10.1016/j.biortech.2019.02.068</a>","BEntID":346888,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Bioresour. Technol. 281</i>: 277-285. <a href=\"https://dx.doi.org/10.1016/j.biortech.2019.02.068\" target=\"_blank\">https://dx.doi.org/10.1016/j.biortech.2019.02.068</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fernandes, H.; Salgado, J.M.; Martins, N.; Peres, H.; Oliva-Teles, A.; Belo, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fernandes, H. <i>et al.</i>","Englishabstract":"The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by <em>Aspergillus ibericus</em> and enzymatic hydrolysis (EH) were applied to washed and unwashed <em>Ulva rigida</em>. SSF of unwashed macroalgae showed higher xylanase (359.8 U/g), cellulase (73.07 U/g) and <em>β</em>-glucosidase (14.9 U/g) activities per dry mass of macroalgae. After SSF, two strategies to carry out EH were assayed. The best process was SSF followed by EH by simply adding a buffer. The non-starch polysaccharides content was reduced by 93.2%, achieving a glucan conversion of 98%. In addition, the antioxidant activity was improved 2.8-fold and the protein concentration of macroalgae extracts increased from 16.9% to 29.8% (w/w). 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