{"refrec":{"BRefID":34993,"RR":"<b>Yugueros, J.; Temprano, A.; Luengo, J.M.; Naharro, G.</b> (2001). Molecular cloning of <i>Yersinia ruckeri</i> aroA gene: a useful taxonomic tool. <i>J. Fish Dis. 24</i>: 383-390. <a href=\"https://dx.doi.org/10.1046/j.1365-2761.2001.00311.x\" target=\"_blank\">https://dx.doi.org/10.1046/j.1365-2761.2001.00311.x</a>","BEntID":34993,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Fish Dis. 24</i>: 383-390. <a href=\"https://dx.doi.org/10.1046/j.1365-2761.2001.00311.x\" target=\"_blank\">https://dx.doi.org/10.1046/j.1365-2761.2001.00311.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yugueros, J.; Temprano, A.; Luengo, J.M.; Naharro, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yugueros, J. <i>et al.</i>","Englishabstract":"The aroA gene of <i>Yersinia ruckeri</i>, which encodes 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase was cloned by complementation of the <i>aroA</i> mutation in <i>Escherichia coli</i> AB2829 by using pUC18 plasmid as a vector. Nucleotide sequence of the <i>aroA</i> gene revealed an open reading frame of 427 amino acids showing a high degree of homology to other bacterial AroA proteins. A pair of primers with 23 and 20 nucleotides were selected from the 5’ and 3’ termini, respectively, and formed the basis of a specific polymerase chain reaction (PCR) assay. A 1165-bp deoxyribonucleic acid (DNA) fragment was amplified from all lysed <i>Y. ruckeri</i> strains. An identical size fragment was also amplified from lysed <i>Y. pseudotuberculosis, Y. aldovae, Salmonella enteritidis</i> and <i>E. coli</i>, but not from other enterobacteria. <i>Alu</i>I restriction fragment length polymorphism (RFLP) of the PCR amplified products allowed for differentiation between <i>Y. ruckeri</i> and the other bacteria. Specificity and sensitivity make this PCR assay a useful method for rapid identification and diagnosis of <i>Y. ruckeri</i> infections.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Molecular cloning of <i>Yersinia ruckeri</i> aroA gene: a useful taxonomic tool","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:02.430286","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2003-05-20","DateCreate":"2003-05-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000170667300002","VABBcode":null,"OpenAcc":0,"DOI":"10.1046/j.1365-2761.2001.00311.x"},"refs":null,"anarec":{"AnaID":34993,"PubliDate":2001,"Pagination":"383-390","XtraPublOfAnaID":null,"ISBN":null,"Volume":"24","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne.  ISSN 0140-7775; e-ISSN 1365-2761","StandardTitleSer":"Journal of Fish Diseases","ISSN":"0140-7775","AbbrevSer":"J. Fish Dis.","StandardTitleMon":null,"StartPage":383,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Yugueros","Firstname":null,"Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34993,"AutID":37412,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Temprano","Firstname":null,"Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34993,"AutID":37413,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Luengo","Firstname":null,"Initials":"J.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34993,"AutID":37414,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Naharro","Firstname":null,"Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":34993,"AutID":37415,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Science","City":"Oxford; London; Edinburgh; Boston; Melbourne"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":34993,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1046/j.1365-2761.2001.00311.x","externalID":"10.1046/j.1365-2761.2001.00311.x","URLTypeCode":"DOI","URLID":111383,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Milh, Dominique, D.","newSesDate":{"date":"2003-05-20 08:54:02.750000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Milh, Dominique, D.","updSesDate":{"date":"2003-05-20 08:54:02.750000","timezone_type":3,"timezone":"Europe/Brussels"}}}