{"refrec":{"BRefID":35001,"RR":"<b>Ardelli, B.F.; Woo, P.T.K.</b> (2001). Conjugation of isometamidium chloride to antibodies and the use of the conjugate against the haemoflagellate, <i>Cryptobia salmositica</i> Katz, 1951: an immuno-chemotherapeutic strategy. <i>J. Fish Dis. 24</i>: 439-451. <a href=\"https://dx.doi.org/10.1046/j.1365-2761.2001.00317.x\" target=\"_blank\">https://dx.doi.org/10.1046/j.1365-2761.2001.00317.x</a>","BEntID":35001,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Fish Dis. 24</i>: 439-451. <a href=\"https://dx.doi.org/10.1046/j.1365-2761.2001.00317.x\" target=\"_blank\">https://dx.doi.org/10.1046/j.1365-2761.2001.00317.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ardelli, B.F.; Woo, P.T.K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ardelli, B.F.; Woo, P.T.K.","Englishabstract":"The trypanocidal drug isometamidium chloride (Samorin) was conjugated to polyclonal and monoclonal antibodies produced against the pathogenic haemoflagellate <i>Cryptobia salmositica</i>. Under in vitro conditions the unconjugated drug normally accumulates rapidly in the kinetoplast in the parasite; however, once it was conjugated to antibodies (either polyclonal or monoclonal) it was found throughout the parasite. Isometamidium conjugated to polyclonal antibodies lysed <i>C. salmositica</i> under in vitro conditions, but parasites were not agglutinated. In contrast, isometamidium conjugated to monoclonal antibodies (against a 200 kDa surface membrane glycoprotein) did not lyse <i>C. salmositica</i>, but parasites were agglutinated. Because of the low efficacy of the monoclonal conjugate against the parasite <i>in vitro</i>, its cryptobiocidal effect was not evaluated further. The infectivity of <i>C. salmositica</i> (incubated either in culture medium or whole blood) was reduced in fish after in vitro exposure to isometamidium conjugated to polyclonal antibodies. Parasitaemias were reduced in infected chinook salmon, <i>Oncorhynchus tshawytscha</i>, after treatment with isometamidium conjugated to polyclonal antibodies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Conjugation of isometamidium chloride to antibodies and the use of the conjugate against the haemoflagellate, <i>Cryptobia salmositica</i> Katz, 1951: an immuno-chemotherapeutic strategy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-25 01:31:29.058468","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2003-05-20","DateCreate":"2003-05-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000171586700002","VABBcode":null,"OpenAcc":0,"DOI":"10.1046/j.1365-2761.2001.00317.x"},"refs":null,"anarec":{"AnaID":35001,"PubliDate":2001,"Pagination":"439-451","XtraPublOfAnaID":null,"ISBN":null,"Volume":"24","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne.  ISSN 0140-7775; e-ISSN 1365-2761","StandardTitleSer":"Journal of Fish Diseases","ISSN":"0140-7775","AbbrevSer":"J. Fish Dis.","StandardTitleMon":null,"StartPage":439,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ardelli","Firstname":"Bernadette","Initials":"B.F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":35001,"AutID":37425,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Woo","Firstname":"Patrick","Initials":"P.T.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":35001,"AutID":37418,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Science","City":"Oxford; London; Edinburgh; Boston; Melbourne"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":35001,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1046/j.1365-2761.2001.00317.x","externalID":"10.1046/j.1365-2761.2001.00317.x","URLTypeCode":"DOI","URLID":111387,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Milh, Dominique, D.","newSesDate":{"date":"2003-05-20 08:54:02.750000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Milh, Dominique, D.","updSesDate":{"date":"2003-05-20 08:54:02.750000","timezone_type":3,"timezone":"Europe/Brussels"}}}