{"refrec":{"BRefID":350623,"RR":"<b>Li, J.; Zhang, C.; Hu, X.; Yoshida, A.; Osatomi, K.; Huo, X.; Yang, J.-L.; Liang, X.</b> (2022). Impact of different enzymes on biofilm formation and mussel settlement. <i>NPG Scientific Reports 12</i>: 4685. <a href=\"https://dx.doi.org/10.1038/s41598-022-08530-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-08530-4</a>","BEntID":348319,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 12</i>: 4685. <a href=\"https://dx.doi.org/10.1038/s41598-022-08530-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-08530-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, J.; Zhang, C.; Hu, X.; Yoshida, A.; Osatomi, K.; Huo, X.; Yang, J.-L.; Liang, X.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, J. <i>et al.</i>","Englishabstract":"Enzymes have been known to impact the biofilm forming capacity. However, how the enzymes mediate the biofilm formation and macrofouling remains little known. Here, we investigated the effects of the three kinds of proteases, four kinds of glycosidases and one kind of lipase on the detachment of biofilms of <i>Shewanella marisflavi</i> ECSMB14101, identified biofilm total proteins response to enzyme treatments, and then tested the effects of biofilms treated with enzymes on the settlement of the mussel <i>Mytilus coruscus</i> plantigrades. The results showed that the cell density of bacteria in biofilms formed at different initial bacterial density were noticeably reduced after treating with all tested enzymes, and Neutrase and α-Amylase exhibited best removing efficiency of &gt; 90%. Bacterial total proteins in <i>S. marisflavi</i> biofilm noticeably reduced or disappeared after treated by Alcalase. For the settlements of the mussel <i>M. coruscus</i> plantigrades, inducing capacities of <i>S. marisflavi</i> biofilm were noticeably suppressed and downregulation was &gt; 75% at the initial density of 5 × 10<sup>6</sup> cells/cm<sup>2</sup>. Thus, the tested enzymes could effectively remove the adhered bacterial cell, inhibit the biofilm formation and finally suppress the mussel settlement. Our findings extend novel knowledge to developing eco-friendly approach to control micro- and macro-fouling.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Impact of different enzymes on biofilm formation and mussel settlement","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:33:41.382985","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-03-24","DateCreate":"2022-03-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000770698200074","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-022-08530-4"},"refs":null,"anarec":{"AnaID":350623,"PubliDate":2022,"Pagination":"4685","XtraPublOfAnaID":null,"ISBN":null,"Volume":"12","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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