{"refrec":{"BRefID":352712,"RR":"<b>Hilal, T.; Killam, B.Y.; Grozdanovic, M.; Dobosz-Bartoszek, M.; Loerke, J.; Bürger, J.; Mielke, T.; Copeland, P.R.; Simonovic, M.; Spahn, C.M.T.</b> (2022). Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon. <i>Science (Wash.) 376(6599)</i>: 1338-1343. <a href=\"https://dx.doi.org/10.1126/science.abg3875\" target=\"_blank\">https://dx.doi.org/10.1126/science.abg3875</a>","BEntID":350420,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Science (Wash.) 376(6599)</i>: 1338-1343. <a href=\"https://dx.doi.org/10.1126/science.abg3875\" target=\"_blank\">https://dx.doi.org/10.1126/science.abg3875</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hilal, T.; Killam, B.Y.; Grozdanovic, M.; Dobosz-Bartoszek, M.; Loerke, J.; Bürger, J.; Mielke, T.; Copeland, P.R.; Simonovic, M.; Spahn, C.M.T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hilal, T. <i>et al.</i>","Englishabstract":"The elongation of eukaryotic selenoproteins relies on a poorly understood process of interpreting in-frame UGA stop codons as selenocysteine (Sec). We used cryo-electron microscopy to visualize Sec UGA recoding in mammals. A complex between the noncoding Sec-insertion sequence (SECIS), SECIS-binding protein 2 (SBP2), and 40<i>S</i> ribosomal subunit enables Sec-specific elongation factor eEFSec to deliver Sec. eEFSec and SBP2 do not interact directly but rather deploy their carboxyl-terminal domains to engage with the opposite ends of the SECIS. By using its Lys-rich and carboxyl-terminal segments, the ribosomal protein eS31 simultaneously interacts with Sec-specific transfer RNA (tRNA<sup>Sec</sup>) and SBP2, which further stabilizes the assembly. eEFSec is indiscriminate toward <span data-style=\"small-caps\">l</span>-serine and facilitates its misincorporation at Sec UGA codons. Our results support a fundamentally distinct mechanism of Sec UGA recoding in eukaryotes from that in bacteria","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:54.200222","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-06-17","DateCreate":"2022-06-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000815052900049","VABBcode":null,"OpenAcc":0,"DOI":"10.1126/science.abg3875"},"refs":null,"anarec":{"AnaID":352712,"PubliDate":2022,"Pagination":"1338-1343","XtraPublOfAnaID":null,"ISBN":null,"Volume":"376","Issue":"6599","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43776,"SerRR":"Science (Washington). 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