{"refrec":{"BRefID":352760,"RR":"<b>An, D.; Pinheiro-Junior, E.L.; Béress, L.; Gladkikh, I.; Leychenko, E.; Undheim, E.A.B.; Peigneur, S.; Tytgat, J.</b> (2022). AsKC11, a Kunitz peptide from <i>Anemonia sulcata</i>, is a novel activator of G protein-coupled inward-rectifier potassium channels. <i>Mar. Drugs 20(2)</i>: 140. <a href=\"https://dx.doi.org/10.3390/md20020140\" target=\"_blank\">https://dx.doi.org/10.3390/md20020140</a>","BEntID":350469,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Drugs 20(2)</i>: 140. <a href=\"https://dx.doi.org/10.3390/md20020140\" target=\"_blank\">https://dx.doi.org/10.3390/md20020140</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"An, D.; Pinheiro-Junior, E.L.; Béress, L.; Gladkikh, I.; Leychenko, E.; Undheim, E.A.B.; Peigneur, S.; Tytgat, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"An, D. <i>et al.</i>","Englishabstract":"(1) Background: G protein-coupled inward-rectifier potassium (GIRK) channels, especially neuronal GIRK1/2 channels, have been the focus of intense research interest for developing drugs against brain diseases. In this context, venom peptides that selectively activate GIRK channels can be seen as a new source for drug development. Here, we report on the identification and electrophysiological characterization of a novel activator of GIRK1/2 channels, AsKC11, found in the venom of the sea anemone <span class=\"html-italic\">Anemonia sulcata</span>. (2) Methods: AsKC11 was purified from the sea anemone venom by reverse-phase chromatography and the sequence was identified by mass spectrometry. Using the two-electrode voltage-clamp technique, the activity of AsKC11 on GIRK1/2 channels was studied and its selectivity for other potassium channels was investigated. (3) Results: AsKC11, a Kunitz peptide found in the venom of <span class=\"html-italic\">A. sulcata</span>, is the first peptide shown to directly activate neuronal GIRK1/2 channels independent from Gi/o protein activity, without affecting the inward-rectifier potassium channel (IRK1) and with only a minor effect on K<sub>V</sub>1.6 channels. Thus, AsKC11 is a novel activator of GIRK channels resulting in larger K<sup>+</sup> currents because of an increased chord conductance. (4) Conclusions: These discoveries provide new insights into a novel class of GIRK activators.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"AsKC11, a Kunitz peptide from <i>Anemonia sulcata</i>, is a novel activator of G protein-coupled inward-rectifier potassium channels","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-05 01:31:27.347681","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"sea anemone venom; AsKC11; GIRK1/2; potassium channels; brain diseases","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-06-22","DateCreate":"2022-06-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000762794100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/md20020140"},"refs":null,"anarec":{"AnaID":352760,"PubliDate":2022,"Pagination":"140","XtraPublOfAnaID":null,"ISBN":null,"Volume":"20","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":113252,"SerRR":"Marine Drugs. 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