{"refrec":{"BRefID":352998,"RR":"<b>Gon\u00e7alves, M.F.M.; Hil\u00e1rio, S.; Tac\u00e3o, M.; Van de Peer, Y.; Alves, A.; Esteves, A.C.<\/b> (2021). Genome and metabolome MS-based mining of a marine strain of <i>Aspergillus affinis<\/i>. <i>Journal of Fungi 7(12)<\/i>: 1091. <a href=\"https:\/\/dx.doi.org\/10.3390\/jof7121091\" target=\"_blank\">https:\/\/dx.doi.org\/10.3390\/jof7121091<\/a>","BEntID":350707,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Journal of Fungi 7(12)<\/i>: 1091. <a href=\"https:\/\/dx.doi.org\/10.3390\/jof7121091\" target=\"_blank\">https:\/\/dx.doi.org\/10.3390\/jof7121091<\/a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Gon\u00e7alves, M.F.M.; Hil\u00e1rio, S.; Tac\u00e3o, M.; Van de Peer, Y.; Alves, A.; Esteves, A.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gon\u00e7alves, M.F.M. <i>et al.<\/i>","Englishabstract":"<i>Aspergillus<\/i> section <i>Circumdati<\/i> encompasses several species that express both beneficial (e.g., biochemical transformation of steroids and alkaloids, enzymes and metabolites) and harmful compounds (e.g., production of ochratoxin A (OTA)). Given their relevance, it is important to analyze the genetic and metabolic diversity of the species of this section. We sequenced the genome of <i>Aspergillus affinis<\/i> CMG 70, isolated from sea water, and compared it with the genomes of species from section <i>Circumdati<\/i>, including <i>A. affinis<\/i>\u2019s strain type. The <i>A. affinis<\/i> genome was characterized considering secondary metabolites biosynthetic gene clusters (BGCs), carbohydrate-active enzymes (CAZymes), and transporters. To uncover the biosynthetic potential of <i>A. affinis<\/i> CMG 70, an untargeted metabolomics (LC-MS\/MS) approach was used. Cultivating the fungus in the presence and absence of sea salt showed that <i>A. affinis<\/i> CMG 70 metabolite profiles are salt dependent. Analyses of the methanolic crude extract revealed the presence of both unknown and well-known <i>Aspergillus<\/i> compounds, such as ochratoxin A, anti-viral (e.g., 3,5-Di-tert-butyl-4-hydroxybenzoic acid and epigallocatechin), anti-bacterial (e.g., 3-Hydroxybenzyl alcohol, l-pyroglutamic acid, lecanoric acid), antifungal (e.g., l-pyroglutamic acid, 9,12,13-Trihydroxyoctadec-10-enoic acid, hydroxyferulic acid), and chemotherapeutic (e.g., daunomycinone, mitoxantrone) related metabolites. Comparative analysis of 17 genomes from 16 <i>Aspergillus<\/i> species revealed abundant CAZymes (568 per species), secondary metabolite BGCs (73 per species), and transporters (1359 per species). Some BGCs are highly conserved in this section (e.g., pyranonigrin E and UNII-YC2Q1O94PT (ACR toxin I)), while others are incomplete or completely lost among species (e.g., bikaverin and chaetoglobosins were found exclusively in series <i>Sclerotiorum<\/i>, while asperlactone seemed completely lost). The results of this study, including genome analysis and metabolome characterization, emphasize the molecular diversity of <i>A. affinis<\/i> CMG 70, as well as of other species in the section <i>Circumdati<\/i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genome and metabolome MS-based mining of a marine strain of <i>Aspergillus affinis<\/i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"antimicrobial; anti-cancer; comparative genomics; marine fungi; metabolites; whole genome sequencing","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2022-10-06","DateCreate":"2022-06-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000738237100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390\/jof7121091"},"refs":null,"anarec":{"AnaID":352998,"PubliDate":2021,"Pagination":"1091","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":347345,"SerRR":"Journal of Fungi. 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