{"refrec":{"BRefID":353029,"RR":"<b>Li, X.; Gong, Y.; Yao, W.; Chen, X.; Xian, J.; You, L.; Fardim, P.</b> (2021). Structural characterization and protective effects of polysaccharide from <i>Gracilaria lemaneiformis</i> on LPS-induced injury in IEC-6 cells. <i>Food Chemistry: X 12</i>: 100157. <a href=\"https://dx.doi.org/10.1016/j.fochx.2021.100157\" target=\"_blank\">https://dx.doi.org/10.1016/j.fochx.2021.100157</a>","BEntID":350738,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Food Chemistry: X 12</i>: 100157. <a href=\"https://dx.doi.org/10.1016/j.fochx.2021.100157\" target=\"_blank\">https://dx.doi.org/10.1016/j.fochx.2021.100157</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, X.; Gong, Y.; Yao, W.; Chen, X.; Xian, J.; You, L.; Fardim, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, X. <i>et al.</i>","Englishabstract":"This study was aimed to characterize <em>Gracilaria lemaneiformis</em> polysaccharides and evaluate their protective effects on Lipopolysaccharide-induced injury in IEC-6 cells. The <em>G. lemaneiformis</em> polysaccharide was degraded by UV/H<sub>2</sub>O<sub>2</sub> treatment and purified to three fractions named GLP-1.0&nbsp;M, GLP-1.4&nbsp;M and GLP-1.6&nbsp;M. The purified fractions were mainly composed of galactose, glucose and xylose. The structural analysis showed that GLP-1.6&nbsp;M was a typical sulfated red alga polysaccharide containing the linear backbone of β-(1&nbsp;→&nbsp;3)- and α-(1&nbsp;→&nbsp;4)-linked galactosyl residues, anhydro-galactose units. In the Lipopolysaccharide-induced IEC-6 cells model, GLP-1.6&nbsp;M exerted the strongest <em>in vitro</em> anti-inflammatory activity by inhibiting the release and expressions of tumor necrosis factor-α, interleukin-6 and interleukin-1β by 89.93%, 67.82% and 38.06%, respectively. Meanwhile, GLP-1.6&nbsp;M enhanced the intestinal barrier function via up-regulating the expressions of tight junctions and mucin. Therefore, the purified polysaccharide from <em>G. lemaneiformis</em> could be a promising candidate for maintaining intestinal health in the food and pharmaceutical industries.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Structural characterization and protective effects of polysaccharide from <i>Gracilaria lemaneiformis</i> on LPS-induced injury in IEC-6 cells","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Gracilaria lemaneiformis; Sulfated polysaccharide; Anti-inflammatory activity; Tight junction","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2022-06-27","DateCreate":"2022-06-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000742031700031","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.fochx.2021.100157"},"refs":null,"anarec":{"AnaID":353029,"PubliDate":2021,"Pagination":"100157","XtraPublOfAnaID":null,"ISBN":null,"Volume":"12","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":353089,"SerRR":"Food Chemistry: X. 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