{"refrec":{"BRefID":353266,"RR":"<b>Thoré, E.S.J.; Schoeters, F.; Spit, J.; Van Miert, S.</b> (2021). Real-time monitoring of microalgal biomass in pilot-scale photobioreactors using nephelometry. <i>Processes 9(9)</i>: 1530. <a href=\"https://dx.doi.org/10.3390/pr9091530\" target=\"_blank\">https://dx.doi.org/10.3390/pr9091530</a>","BEntID":350975,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Processes 9(9)</i>: 1530. <a href=\"https://dx.doi.org/10.3390/pr9091530\" target=\"_blank\">https://dx.doi.org/10.3390/pr9091530</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Thoré, E.S.J.; Schoeters, F.; Spit, J.; Van Miert, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Thoré, E.S.J. <i>et al.</i>","Englishabstract":"The increasing cultivation of microalgae in photobioreactors warrants efficient and non-invasive methods to quantify biomass density in real time. Nephelometric turbidity assessment, a method that measures light scatter by particles in suspension, was introduced already several decades ago but was only recently validated as a high-throughput tool to monitor microalgae biomass. The light scatter depends on the density of the suspended particles as well as on their physical properties, but so far there are hardly any accounts on how nephelometric assessment relates to classic methods such as dry weight and spectrophotometric measurement across a broad biomass density range for different microalgae species. Here, we monitored biomass density online and in real time during the semi-continuous cultivation of three commercial microalgae species <i>Chloromonas typhlos</i>, <i>Microchloropsis gaditana</i> and <i>Porphyridium purpureum</i> in pilot-scale photobioreactors, and relate nephelometric turbidity to dry weight and optical density. The results confirm a relatively strong (R<sup>2</sup> = 0.87–0.93) and nonlinear relationship between turbidity and biomass density that differs among the three species. Overall, we demonstrate how nephelometry can be used to monitor microalgal biomass in photobioreactors, and provide the necessary means to estimate the biomass density of the studied species from turbidity data to facilitate automated biomass monitoring.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Real-time monitoring of microalgal biomass in pilot-scale photobioreactors using nephelometry","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"microalgae; algal cultivation; biotechnology; turbidity; cell density; NTU; automation","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2023-06-22","DateCreate":"2022-07-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000701948100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/pr9091530"},"refs":null,"anarec":{"AnaID":353266,"PubliDate":2021,"Pagination":"1530","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":276791,"SerRR":"Processes. MDPI AG: Basel.  ISSN 2227-9717; e-ISSN 2227-9717","StandardTitleSer":"Processes","ISSN":"2227-9717","AbbrevSer":null,"StandardTitleMon":null,"StartPage":9,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Thoré","Firstname":"Eli","Initials":"E.S.J.","Affiliation":"Radius, Thomas More University of Applied Sciences","Discriminator":null,"CorporateFlag":0,"BEntID":350975,"AutID":493121,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Thomas More; Radius","ORCID":"0000-0002-0029-8404","PersID":41343,"InsID":15209},{"AutName":"Schoeters","Firstname":"Floris","Initials":"F.","Affiliation":"Radius, Thomas More University of Applied Sciences","Discriminator":null,"CorporateFlag":0,"BEntID":350975,"AutID":493122,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Thomas More; Radius","ORCID":"0000-0003-2744-8756","PersID":43698,"InsID":15209},{"AutName":"Spit","Firstname":"Jornt","Initials":"J.","Affiliation":"Radius, Thomas More University of Applied Sciences","Discriminator":null,"CorporateFlag":0,"BEntID":350975,"AutID":493123,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Thomas More; Radius","ORCID":"0000-0002-3110-7148","PersID":43718,"InsID":15209},{"AutName":"Van Miert","Firstname":"Sabine","Initials":"S.","Affiliation":"Radius, Thomas More University of Applied Sciences","Discriminator":null,"CorporateFlag":0,"BEntID":350975,"AutID":493124,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Thomas More; Radius","ORCID":"0000-0003-2328-3130","PersID":43717,"InsID":15209}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":378537,"LibID":36,"BRefID":353266,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"378537","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"MDPI AG","City":"Basel"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Microchloropsis gaditana"},{"TaxTerm":"Porphyridium purpureum"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":350975,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":350975,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.3390/pr9091530","externalID":"10.3390/pr9091530","URLTypeCode":"DOI","URLID":103983,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Microchloropsis gaditana","AphiaID":1370287,"TaxtID":52300},{"TaxTerm":"Porphyridium purpureum","AphiaID":145788,"TaxtID":49050}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Microchloropsis gaditana","DutchTerm":null,"AphiaID":1370287,"TaxtID":52300},{"TaxTerm":"Porphyridium purpureum","DutchTerm":null,"AphiaID":145788,"TaxtID":49050}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2022-07-01 14:21:55.743000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lust, Heike, H.","updSesDate":{"date":"2023-06-22 13:02:23.140000","timezone_type":3,"timezone":"Europe/Brussels"}}}