{"refrec":{"BRefID":354190,"RR":"<b>You, Y.; Sun, X.; Ma, M.; He, J.; Li, L.; Porto, F.W.; Lin, S.</b> (2022). Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton. <i>Nature Comm. 13(1)</i>: 4022. <a href=\"https://dx.doi.org/10.1038/s41467-022-31802-6\" target=\"_blank\">https://dx.doi.org/10.1038/s41467-022-31802-6</a>","BEntID":351903,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Nature Comm. 13(1)</i>: 4022. <a href=\"https://dx.doi.org/10.1038/s41467-022-31802-6\" target=\"_blank\">https://dx.doi.org/10.1038/s41467-022-31802-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"You, Y.; Sun, X.; Ma, M.; He, J.; Li, L.; Porto, F.W.; Lin, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"You, Y. <i>et al.</i>","Englishabstract":"Trypsin is best known as a digestive enzyme in animals, but remains unexplored in phytoplankton, the major primary producers in the ocean. Here we report the prevalence of trypsin genes in global ocean phytoplankton and significant influences of environmental nitrogen (N) and phosphorus (P) on their expression. Using <i>CRISPR/Cas9</i> mediated<i>-</i>knockout and overexpression analyses, we further reveal that a trypsin in <i>Phaeodactylum tricornutum</i> (<i>PtTryp2</i>) functions to repress N acquisition, but its expression decreases under N-deficiency to promote N acquisition. On the contrary, <i>PtTryp2</i> promotes phosphate uptake per se, and its expression increases under P-deficiency to further reinforce P acquisition. Furthermore, <i>PtTryp2</i> knockout led to amplitude magnification of the nitrate and phosphate uptake ‘seesaw’, whereas <i>PtTryp2</i> overexpression dampened it, linking <i>PtTryp2</i> to stabilizing N:P stoichiometry. Our data demonstrate that <i>PtTryp2</i> is a coordinate regulator of N:P stoichiometric homeostasis. 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