{"refrec":{"BRefID":354614,"RR":"<b>Koppelle, S.; López-Escardó, D.; Brussaard, C.P.D.; Huisman, J.; Philippart, C.J.M.; Massana, R.; Wilken, S.</b> (2022). Mixotrophy in the bloom-forming genus <i>Phaeocystis</i> and other haptophytes. <i>Harmful Algae 117</i>: 102292. <a href=\"https://dx.doi.org/10.1016/j.hal.2022.102292\" target=\"_blank\">https://dx.doi.org/10.1016/j.hal.2022.102292</a>","BEntID":352327,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Harmful Algae 117</i>: 102292. <a href=\"https://dx.doi.org/10.1016/j.hal.2022.102292\" target=\"_blank\">https://dx.doi.org/10.1016/j.hal.2022.102292</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Koppelle, S.; López-Escardó, D.; Brussaard, C.P.D.; Huisman, J.; Philippart, C.J.M.; Massana, R.; Wilken, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Koppelle, S. <i>et al.</i>","Englishabstract":" <i>Phaeocystis</i>    is a globally widespread marine phytoplankton genus, best known for its    colony-forming species that can form large blooms and odorous foam during    bloom decline. In the North Sea, <i>Phaeocystis globosa</i> typically    becomes abundant towards the end of the spring bloom, when nutrients are    depleted and the share of mixotrophic protists increases. Although    mixotrophy is widespread across the eukaryotic tree of life and is also    found amongst haptophytes, a mixotrophic nutrition has not yet beendemonstrated in <i>Phaeocystis</i>. Here, we sampled two consecutive    <i>Phaeocystis globosa</i> spring blooms in the coastal North Sea. In both    years, bacterial cells were observed inside 0.6 – 2% of <i>P. globosa</i>    cells using double CARD-FISH hybridizations in combination with laser    scanning confocal microscopy. Incubation experiments manipulating light and    nutrient availability showed a trend towards higher occurrence of    intracellular bacteria under P-deplete conditions. Based on counts of    bacteria inside <i>P. globosa</i> cells in combination with theoretical    values of prey digestion times, maximum ingestion rates of up to 0.08    bacteria cell<sup>−1</sup> h<sup>−1</sup> were estimated. In addition, a    gene-based predictive model was applied to the transcriptome assemblies of    seven <i>Phaeocystis</i> strains and 24 other haptophytes to assess their    trophic mode. This model predicted a phago-mixotrophic feeding strategy in    several (but not all) strains of <i>P. globosa, P. antarctica</i> and other    haptophytes that were previously assumed to be autotrophic. The observation    of bacterial cells inside <i>P. globosa</i> and the gene-based model    predictions strongly suggest that the phago-mixotrophic feeding strategy is    widespread among members of the <i>Phaeocystis</i> genus and other    haptophytes, and might contribute to their remarkable success to form    nuisance blooms under nutrient-limiting conditions.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Mixotrophy in the bloom-forming genus <i>Phaeocystis</i> and other haptophytes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Phaeocystis globosa; Mixotrophy; Bacterivory; Confocal microscopy; Haptophyte","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-10-05","DateCreate":"2022-08-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000844391200004","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.hal.2022.102292"},"refs":null,"anarec":{"AnaID":354614,"PubliDate":2022,"Pagination":"102292","XtraPublOfAnaID":null,"ISBN":null,"Volume":"117","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45642,"SerRR":"Harmful Algae. 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