{"refrec":{"BRefID":356379,"RR":"<b>Faktorová, Drahomíra; Nisbet, R. Ellen R.; Fernández Robledo, José A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Aresté, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, François-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, Pía A.; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J.; Fukuda, Kodai; García, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladimír; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Ibañez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa; Jones, Adam C.; Kachale, Ambar; Fujimura-Kamada, Konomi; Kaur, Binnypreet; Kaye, Jonathan Z.; Kazana, Eleanna; Keeling, Patrick J.; King, Nicole; Klobutcher, Lawrence A.; Lander, Noelia; Lassadi, Imen; Li, Zhuhong; Lin, Senjie; Lozano, Jean-Claude; Luan, Fulei; Maruyama, Shinichiro; Matute, Tamara; Miceli, Cristina; Minagawa, Jun; Moosburner, Mark; Najle, Sebastián R.; Nanjappa, Deepak; Nimmo, Isabel C.; Noble, Luke; Novák Vanclová, Anna M. G.; Nowacki, Mariusz; Nuñez, Isaac; Pain, Arnab; Piersanti, Angela; Pucciarelli, Sandra; Pyrih, Jan; Rest, Joshua S.; Rius, Mariana; Robertson, Deborah; Ruaud, Albane; Ruiz-Trillo, Iñaki; Sigg, Monika A.; Silver, Pamela A.; Slamovits, Claudio H.; Jason Smith, G.; Sprecher, Brittany N.; Stern, Rowena; Swart, Estienne C.; Tsaousis, Anastasios D.; Tsypin, Lev; Turkewitz, Aaron; Turnšek, Jernej; Valach, Matus; Vergé, Valérie; von Dassow, Peter; von der Haar, Tobias; Waller, Ross F.; Wang, Lu; Wen, Xiaoxue; Wheeler, Glen; Woods, April; Zhang, Huan; Mock, Thomas; Worden, Alexandra Z.; Lukeš, Julius</b> (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. <i>Nature Methods 17(5)</i>: 481-494. <a href=\"https://dx.doi.org/10.1038/s41592-020-0796-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41592-020-0796-x</a>","BEntID":354093,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Nature Methods 17(5)</i>: 481-494. <a href=\"https://dx.doi.org/10.1038/s41592-020-0796-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41592-020-0796-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Faktorová, Drahomíra; Nisbet, R. Ellen R.; Fernández Robledo, José A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Aresté, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, François-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, Pía A.; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J.; Fukuda, Kodai; García, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladimír; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Ibañez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa; Jones, Adam C.; Kachale, Ambar; Fujimura-Kamada, Konomi; Kaur, Binnypreet; Kaye, Jonathan Z.; Kazana, Eleanna; Keeling, Patrick J.; King, Nicole; Klobutcher, Lawrence A.; Lander, Noelia; Lassadi, Imen; Li, Zhuhong; Lin, Senjie; Lozano, Jean-Claude; Luan, Fulei; Maruyama, Shinichiro; Matute, Tamara; Miceli, Cristina; Minagawa, Jun; Moosburner, Mark; Najle, Sebastián R.; Nanjappa, Deepak; Nimmo, Isabel C.; Noble, Luke; Novák Vanclová, Anna M. G.; Nowacki, Mariusz; Nuñez, Isaac; Pain, Arnab; Piersanti, Angela; Pucciarelli, Sandra; Pyrih, Jan; Rest, Joshua S.; Rius, Mariana; Robertson, Deborah; Ruaud, Albane; Ruiz-Trillo, Iñaki; Sigg, Monika A.; Silver, Pamela A.; Slamovits, Claudio H.; Jason Smith, G.; Sprecher, Brittany N.; Stern, Rowena; Swart, Estienne C.; Tsaousis, Anastasios D.; Tsypin, Lev; Turkewitz, Aaron; Turnšek, Jernej; Valach, Matus; Vergé, Valérie; von Dassow, Peter; von der Haar, Tobias; Waller, Ross F.; Wang, Lu; Wen, Xiaoxue; Wheeler, Glen; Woods, April; Zhang, Huan; Mock, Thomas; Worden, Alexandra Z.; Lukeš, Julius","OrigTitleTranslFlag":0,"Authorstringtrunc":"Faktorová, Drahomíra <i>et al.</i>","Englishabstract":"Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genetic tool development in marine protists: emerging model organisms for experimental cell biology","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2022-10-13","DateCreate":"2022-10-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000523954200003","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41592-020-0796-x"},"refs":null,"anarec":{"AnaID":356379,"PubliDate":2020,"Pagination":"481-494","XtraPublOfAnaID":null,"ISBN":null,"Volume":"17","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":275192,"SerRR":"Nature Methods. Nature Publishing Group: London.  ISSN 1548-7091; e-ISSN 1548-7105","StandardTitleSer":"Nature Methods","ISSN":"1548-7091","AbbrevSer":null,"StandardTitleMon":null,"StartPage":481,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":[{"XBRefID":356380,"RefStrFull":"<b>Brown, M.W.; Tice, A.K.</b> (2020). A genetic toolbox for marine protists. <i>Nature Methods 17(5)</i>: 469-470. <a href=\"https://dx.doi.org/10.1038/s41592-020-0794-z\" target=\"_blank\">https://dx.doi.org/10.1038/s41592-020-0794-z</a>","RelID":4,"RelationY":"Related to","StandardTitle":"A genetic toolbox for marine protists"}],"addrec":null,"othpubs":null,"ownerships":null,"authors":null,"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":354093,"BEntPrivate":null,"availability":[{"BInstID":382208,"LibID":36,"BRefID":356379,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"382208","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":952,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 3","ThestID":181465,"Acronym":"KTPKO","ThesTermPath":"ASSEMBLEPlus Joint Research Activity 3"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTermPath":"Scientific Publication"}],"thestermsASFA":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 3"},{"ThesaurusTerm":"Scientific Publication"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"952":{"SpName":"AssemblePlus","SpColID":952,"ParSpColID":990,"TopParID":990,"ShortName":"A+OR","URLLocation":"https://www.assembleplus.eu/assemble-plus-open-repository?module=ref&refid=","LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/A+OR"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":354093,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":354093,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41592-020-0796-x","externalID":"10.1038/s41592-020-0796-x","URLTypeCode":"DOI","URLID":106486,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 3","ThestID":181465,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 3","DutchTerm":null,"ThestID":181465,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Scientific Publication","DutchTerm":null,"ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2022-10-13 08:40:00.413000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2022-10-13 08:40:00.413000","timezone_type":3,"timezone":"Europe/Brussels"}}}