{"refrec":{"BRefID":356412,"RR":"<b>McDougall, A.; Hebras, C.; Gomes, I.; Dumollard, R.</b> (2021). Gene editing in the ascidian <i>Phallusia mammillata</i> and tail nerve cord formation, <b><i>in</i></b>: Carroll, D.J. <i>et al.</i> <i>Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols. Methods in Molecular Biology,</i> 2219: pp. 217-230. <a href=\"https://dx.doi.org/10.1007/978-1-0716-0974-3_13\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-0974-3_13</a>","BEntID":354126,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Carroll, D.J. <i>et al.</i> <i>Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols. Methods in Molecular Biology,</i> 2219: pp. 217-230. <a href=\"https://dx.doi.org/10.1007/978-1-0716-0974-3_13\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-0974-3_13</a>","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"McDougall, A.; Hebras, C.; Gomes, I.; Dumollard, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"McDougall, A. <i>et al.</i>","Englishabstract":"Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian <i>Phallusia mammillata</i>.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Gene editing in the ascidian <i>Phallusia mammillata</i> and tail nerve cord formation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ascidian; Gene editing; CRISPR-Cas9; Microinjection; Nerve cord","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2022-10-14","DateCreate":"2022-10-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0,"DOI":"10.1007/978-1-0716-0974-3_13"},"refs":null,"anarec":{"AnaID":356412,"PubliDate":2021,"Pagination":"217-230","XtraPublOfAnaID":null,"ISBN":"978-1-0716-0976-7","Volume":"2219","Issue":null,"BRefMon":356411,"BRefMonRR":"<b>Carroll, D.J.; Stricker, S.A. (Ed.)</b> (2021). Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols. Second Edition. <i>Methods in Molecular Biology</i>, 2219. Humana Press: New York. ISBN 978-1-0716-0976-7. XIII, 309 pp. <a href=\"https://dx.doi.org/10.1007/978-1-0716-0974-3\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-0974-3</a>","BRefXtra":null,"BRefXtraRR":null,"SerBRefID":294813,"SerRR":"Methods in Molecular Biology. Humana Press.  ISSN 1064-3745","StandardTitleSer":"Methods in Molecular Biology","ISSN":"1064-3745","AbbrevSer":null,"StandardTitleMon":"Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols","StartPage":217,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"MS","SerNotes":null,"AutString":"Carroll, D.J.; Stricker, S.A. (Ed.)"},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"McDougall","Firstname":"Alex","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":354126,"AutID":506335,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hebras","Firstname":"Celine","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":354126,"AutID":506336,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gomes","Firstname":"Isa","Initials":"I.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":354126,"AutID":506337,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dumollard","Firstname":"Rémi","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":354126,"AutID":252698,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":354126,"BEntPrivate":null,"availability":[{"BInstID":382241,"LibID":36,"BRefID":356412,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"382241","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":952,"SERpubls":null,"MONpubls":[{"PublName":"Humana Press","Place":"New York"}],"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Phallusia mammillata"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"952":{"SpName":"AssemblePlus","SpColID":952,"ParSpColID":990,"TopParID":990,"ShortName":"A+OR","URLLocation":"https://www.assembleplus.eu/assemble-plus-open-repository?module=ref&refid=","LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/A+OR"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":[{"AutName":"Carroll","Initials":"D.J.","CorporateFlag":0,"BEntID":354125,"AutID":506334,"OrderNr":1,"DegrID":null,"EditorFlag":1,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"AutStrTrunc":"Carroll, D.J.; Stricker, S.A. (Ed.)"},{"AutName":"Stricker","Initials":"S.A.","CorporateFlag":0,"BEntID":354125,"AutID":23232,"OrderNr":2,"DegrID":null,"EditorFlag":1,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"AutStrTrunc":"Carroll, D.J.; Stricker, S.A. (Ed.)"}],"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":354126,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":354126,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/978-1-0716-0974-3_13","externalID":"10.1007/978-1-0716-0974-3_13","URLTypeCode":"DOI","URLID":106508,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Phallusia mammillata","AphiaID":103724,"TaxtID":53130}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Phallusia mammillata","DutchTerm":null,"AphiaID":103724,"TaxtID":53130}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2022-10-14 08:44:42.357000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2022-10-14 08:44:42.357000","timezone_type":3,"timezone":"Europe/Brussels"}}}