{"refrec":{"BRefID":35770,"RR":"<b>Runcie, J.W.; Ritchie, R.J.; Larkum, A.W.D.</b> (2003). Uptake kinetics and assimilation of inorganic nitrogen by <i>Catenella nipae</i> and <i>Ulva lactuca</i>. <i>Aquat. Bot. 76(2)</i>: 155-174. <a href=\"http://dx.doi.org/10.1016/s0304-3770(03)00037-8\" target=\"_blank\">http://dx.doi.org/10.1016/s0304-3770(03)00037-8</a>","BEntID":35770,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Bot. 76(2)</i>: 155-174. <a href=\"https://dx.doi.org/10.1016/s0304-3770(03)00037-8\" target=\"_blank\">https://dx.doi.org/10.1016/s0304-3770(03)00037-8</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Runcie, J.W.; Ritchie, R.J.; Larkum, A.W.D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Runcie, J.W. <i>et al.</i>","Englishabstract":"The kinetics of NH<sub>4</sub><sup>+</sup>, the assimilation of NH<sub>4</sub><sup>+</sup> and nitrate uptake by <i>Catenella nipae</i> (Rhodophyta) were compared with <i>Ulva lactuca</i> (Chlorophyta). Both algal species demonstrated saturable NH<sub>4</sub><sup>+</sup> and nitrate uptake kinetics. Uptake of NH<sub>3</sub> by simple diffusion across the plasmalemma could not account for the observed saturation uptake kinetics of ammonia-N (NH<sub>3</sub>+ NH<sub>4</sub><sup>+</sup>), so NH<sub>4</sub><sup>+</sup> was the chemical form being taken up by the transport systems of the cells. Although the Vmax of NH<sub>4</sub><sup>+</sup> uptake by <i>C. nipae</i> and <i>U. lactuca</i> was high (550 and 450 µmol g<sup>-1</sup> DW h<sup>-1</sup>, respectively), the Km for <i>U. lactuca</i> (85 µM) was much lower than that for <i>C. nipae</i> (692 µM). The Km and Vmax values for nitrate uptake were much lower than for NH4+ for both <i>C. nipae</i> (Km about 5 µM; Vmax about 8.3 µmol g<sup>-1</sup> DW h<sup>-1</sup>) and <i>U. lactuca</i> (Km about 34 µM; Vmax about 116 µmol g<sup>-1</sup> DW h<sup>-1</sup>). Over the incubation times used (up to 28 min) there was no apparent induction of nitrate transport in either species. There was no evidence for induction of NH<sub>4</sub><sup>+</sup> transport in <i>C. nipae</i> but incubation time did affect the kinetics of NH<sub>4</sub><sup>+</sup> uptake in <i>U. lactuca</i>. At high concentrations of NH<sub>4</sub><sup>+</sup>, <i>U. lactuca</i> rapidly assimilated it into organic N with limited build-up of intracellular NH<sub>4</sub><sup>+</sup> whereas <i>C. nipae</i> accumulated large amounts of NH<sub>4</sub><sup>+</sup> because uptake of NH<sub>4</sub><sup>+</sup> overtook the rate of assimilation. The effects of species-specific differences and experimental design on uptake-kinetic estimates are discussed in the light of the results of this other comparable studies. <i>C. nipae</i> is promising as a bioindicator species of the N-status of estuaries but <i>U. lactuca</i> changes its N-status too quickly for it to be a useful bioindicator of environmental conditions.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Uptake kinetics and assimilation of inorganic nitrogen by <i>Catenella nipae</i> and <i>Ulva lactuca</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:58:19.190595","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"ammonia-N; NH4+; nitrate; assimilation; Ulva lactuca; Catenella nipae;uptake kinetics; bioindicator species","OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2003-06-19","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000183585700006","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/s0304-3770(03)00037-8"},"refs":null,"anarec":{"AnaID":35770,"PubliDate":2003,"Pagination":"155-174","XtraPublOfAnaID":null,"ISBN":null,"Volume":"76","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42195,"SerRR":"Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam.  ISSN 0304-3770; e-ISSN 1879-1522","StandardTitleSer":"Aquatic Botany","ISSN":"0304-3770","AbbrevSer":"Aquat. 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