{"refrec":{"BRefID":359623,"RR":"<b>Harding, K.J.; Turk-Kubo, K.A.; Mak, E.W.K.; Weber, P.K.; Mayali, X.; Zehr, J.P.</b> (2022). Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre. <i>Nature Comm. 13(1)</i>: 6979 . <a href=\"https://dx.doi.org/10.1038/s41467-022-34585-y\" target=\"_blank\">https://dx.doi.org/10.1038/s41467-022-34585-y</a>","BEntID":357338,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Nature Comm. 13(1)</i>: 6979. <a href=\"https://dx.doi.org/10.1038/s41467-022-34585-y\" target=\"_blank\">https://dx.doi.org/10.1038/s41467-022-34585-y</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Harding, K.J.; Turk-Kubo, K.A.; Mak, E.W.K.; Weber, P.K.; Mayali, X.; Zehr, J.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Harding, K.J. <i>et al.</i>","Englishabstract":"Biological nitrogen fixation is a major important source of nitrogen for low-nutrient surface oceanic waters. Nitrogen-fixing (diazotrophic) cyanobacteria are believed to be the primary contributors to this process, but the contribution of non-cyanobacterial diazotrophic organisms in oxygenated surface water, while hypothesized to be important, has yet to be demonstrated. In this study, we used simultaneous <sup>15</sup>N-dinitrogen and <sup>13</sup>C-bicarbonate incubations combined with nanoscale secondary ion mass spectrometry analysis to screen tens of thousands of mostly particle-associated, cell-like regions of interest collected from the North Pacific Subtropical Gyre. These dual isotope incubations allow us to distinguish between non-cyanobacterial and cyanobacterial nitrogen-fixing microorganisms and to measure putative cell-specific nitrogen fixation rates. With this approach, we detect nitrogen fixation by putative non-cyanobacterial diazotrophs in the oxygenated surface ocean, which are associated with organic-rich particles (&lt;210 µm size fraction) at two out of seven locations sampled. When present, up to 4.1% of the analyzed particles contain at least one active putative non-cyanobacterial diazotroph. The putative non-cyanobacterial diazotroph nitrogen fixation rates (0.76 ± 1.60 fmol N cell<sup>−1</sup> d<sup>−1</sup>) suggest that these organisms are capable of fixing dinitrogen in oxygenated surface water, at least when attached to particles, and may contribute to oceanic nitrogen&nbsp;fixation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-09 01:32:28.552577","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2022-12-05","DateCreate":"2022-12-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000884426700009","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41467-022-34585-y"},"refs":null,"anarec":{"AnaID":359623,"PubliDate":2022,"Pagination":"6979","XtraPublOfAnaID":null,"ISBN":null,"Volume":"13","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":198717,"SerRR":"Nature Communications. 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