{"refrec":{"BRefID":359914,"RR":"<b>Ruginescu, R.; Lavin, P.; Iancu, L.; Menabit, S.; Purcarea, C.</b> (2022). Bioprospecting for novel bacterial sources of hydrolytic enzymes and antimicrobials in the Romanian littoral zone of the Black Sea. <i>Microorganisms 10(12)</i>: 2468. <a href=\"https://dx.doi.org/10.3390/microorganisms10122468\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms10122468</a>","BEntID":357629,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Microorganisms 10(12)</i>: 2468. <a href=\"https://dx.doi.org/10.3390/microorganisms10122468\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms10122468</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ruginescu, R.; Lavin, P.; Iancu, L.; Menabit, S.; Purcarea, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ruginescu, R. <i>et al.</i>","Englishabstract":"Marine microorganisms have evolved a large variety of metabolites and biochemical processes, providing great opportunities for biotechnologies. In the search for new hydrolytic enzymes and antimicrobial compounds with enhanced characteristics, the current study explored the diversity of cultured and uncultured marine bacteria in Black Sea water from two locations along the Romanian coastline. Microbial cell density in the investigated samples varied between 65 and 12.7 × 10<sup>3</sup> CFU·mL<sup>−1</sup>. The total bacterial community identified by Illumina sequencing of 16S rRNA gene comprised 185 genera belonging to 46 classes, mainly Gammaproteobacteria, Alphaproteobacteria, Flavobacteriia, and 24 phyla. The 66 bacterial strains isolated on seawater-based culture media belonged to 33 genera and showed variable growth temperatures, growth rates, and salt tolerance. A great fraction of these strains, including <i>Pseudoalteromonas</i> and <i>Flavobacterium</i> species, produced extracellular proteases, lipases, and carbohydrases, while two strains belonging to the genera <i>Aquimarina</i> and <i>Streptomyces</i> exhibited antimicrobial activity against human pathogenic bacteria. This study led to a broader view on the diversity of microbial communities in the Black Sea, and provided new marine strains with hydrolytic and antimicrobial capabilities that may be exploited in industrial and pharmaceutical applications.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Bioprospecting for novel bacterial sources of hydrolytic enzymes and antimicrobials in the Romanian littoral zone of the Black Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"marine bacteria; seawater microbial diversity; Illumina sequencing; halophilic bacteria; extracellular hydrolases; lipase; carbohydrate-degrading enzymes; antimicrobial activity","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-01-04","DateCreate":"2023-01-04","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000903486100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/microorganisms10122468"},"refs":null,"anarec":{"AnaID":359914,"PubliDate":2022,"Pagination":"2468","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":302866,"SerRR":"Microorganisms. 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