Document of bibliographic reference 360067

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Effects of ovarian parasite Marteilioides chungmuensis on the reproduction of the Pacific oyster Crassostrea gigas assessed by histology and enzyme-linked immunosorbent assay (ELISA)

Abstract

The paramyxean parasite Marteilioides chungmuensis infects the cytoplasm of the eggs of Pacific oysters Crassostrea gigas, resulting in spawning failure of the infected females. Such infected eggs appear as bump-like nodules on the body in late fall when most of the uninfected females complete spawning. In this study, we estimated the quantity of the infected eggs using an enzyme-linked immunosorbent assay (ELISA), which is destroyed by M. chungmuensis parasitism. In December, the infected oysters collected from Tongyoung on the south coast exhibited numerous yellowish bump-like nodules as signs of infection. In histology, the infected oysters exhibited mature eggs in the follicle, which were heavily infiltrated by hemocytes. ELISA indicated that the infected egg mass accounted for 7.52±5.50 percent of the body weight, suggesting the ovarian parasite causes substantial reproductive loss. Histology also indicated that the infected oysters are in a poor nutritional condition, as the digestive gland atrophy (DGA) level is comparatively higher than the uninfected oyster. The total carbohydrate contents in the infected oysters (108.68±44.41 mg/g dry wt) were significantly lower than in uninfected oysters (269.76±50.97 mg/g dry wt), suggesting that M. chungmuensis parasitism also affected the energy storage capacity of the host during the resting stage.

Bibliographic citation

Limpanont, Y.; Kang, H.-S.; Cho, Y.-G.; Shin, J.S.; Kajino, N.; Kim, J.-H.; Hong, H.-K.; Choi, K.-S. (2022). Effects of ovarian parasite Marteilioides chungmuensis on the reproduction of the Pacific oyster Crassostrea gigas assessed by histology and enzyme-linked immunosorbent assay (ELISA). Ocean and Polar Research 44(4): 319-329. https://dx.doi.org/10.4217/OPR.2022023

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true