{"refrec":{"BRefID":360955,"RR":"<b>Healey, E.M.; Flood, S.; Bock, P.K.; Fulweiler, R.W.; York, J.K.; Coyne, K.J.</b> (2023). Effects of nitrate and ammonium on assimilation of nitric oxide by <i>Heterosigma akashiwo</i>. <i>NPG Scientific Reports 13(1)</i>: 621. <a href=\"https://dx.doi.org/10.1038/s41598-023-27692-3\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-023-27692-3</a>","BEntID":358670,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 13(1)</i>: 621. <a href=\"https://dx.doi.org/10.1038/s41598-023-27692-3\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-023-27692-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Healey, E.M.; Flood, S.; Bock, P.K.; Fulweiler, R.W.; York, J.K.; Coyne, K.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Healey, E.M. <i>et al.</i>","Englishabstract":"The harmful alga <i>Heterosigma akashiwo</i> possesses a hybrid nitrate reductase (NR) enzyme, NR2-2/2HbN, which has the potential to convert NO to nitrate for assimilation into biomass. In previous research, <i>NR</i> transcription in <i>H. akashiwo</i> was induced by nitrate while NR activity was inhibited by ammonium. Here, the capacity of <i>H. akashiwo</i> to use NO in the presence of nitrate and/or ammonium was investigated to understand the regulation of NO assimilation. Continuous cultures of <i>H. akashiwo</i> were acclimated to growth on nitrate, ammonium, or a mixture of both. Aliquots from these cultures were spiked with <sup>15</sup>N-labeled NO. The expression of genes involved in nitrogen assimilation was evaluated, as well as nitrate reductase activity and assimilation of <sup>15</sup>N-labeled nitrogen into algal biomass. Results showed that NO induced expression and activity of NR, and upregulated expression of <i>GOGAT</i> regardless of the presence of other inorganic nitrogen sources, while <i>GS</i> expression decreased over time. Furthermore, <sup>15</sup>NO uptake and assimilation was significantly higher in cultures acclimated for growth on ammonium compared to cultures acclimated for growth on nitrate alone. Assimilation of NO may provide <i>H. akashiwo</i> with a competitive advantage in N-poor environments or areas with elevated NO.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of nitrate and ammonium on assimilation of nitric oxide by <i>Heterosigma akashiwo</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-12 01:34:33.499866","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-02-02","DateCreate":"2023-02-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000962604100051","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-023-27692-3"},"refs":null,"anarec":{"AnaID":360955,"PubliDate":2023,"Pagination":"621","XtraPublOfAnaID":null,"ISBN":null,"Volume":"13","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":621,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Healey","Firstname":"Emily","Initials":"E.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":514352,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Flood","Firstname":"Stacie","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":514353,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bock","Firstname":"Patience","Initials":"P.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":514354,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Fulweiler","Firstname":"Robinson","Initials":"R.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":405638,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"York","Firstname":"Joanna","Initials":"J.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":514356,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Coyne","Firstname":"Kathryn","Initials":"K.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":358670,"AutID":290902,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":386529,"LibID":36,"BRefID":360955,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"386529","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":358670,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":358670,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-023-27692-3","externalID":"10.1038/s41598-023-27692-3","URLTypeCode":"DOI","URLID":109361,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2023-02-02 08:22:42.983000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2023-02-02 08:22:42.983000","timezone_type":3,"timezone":"Europe/Brussels"}}}