{"refrec":{"BRefID":361114,"RR":"<b>Goudriaan, M.; Hernando-Morales, V.; van der Meer, M.T.J.; Mets, A.; Ndhlovu, R.; van Heerwaarden, J.; Simon, S.; Heuer, V.B.; Hinrichs, K.-U.; Niemann, H.</b> (2023). A stable isotope assay with 13C-labeled polyethylene to investigate plastic mineralization mediated by Rhodococcus ruber. <i>Mar. Pollut. Bull. 186</i>: 114369. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2022.114369\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2022.114369</a>","BEntID":358829,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mar. Pollut. Bull. 186</i>: 114369. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2022.114369\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2022.114369</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Goudriaan, M.; Hernando-Morales, V.; van der Meer, M.T.J.; Mets, A.; Ndhlovu, R.; van Heerwaarden, J.; Simon, S.; Heuer, V.B.; Hinrichs, K.-U.; Niemann, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Goudriaan, M. <i>et al.</i>","Englishabstract":"<p>    Methods that unambiguously prove microbial plastic degradation and allow    for quantification of degradation rates are necessary to constrain the    influence of microbial degradation on the marine plastic budget. We    developed an assay based on stable isotope tracer techniques to determine    microbial plastic mineralization rates in liquid medium on a lab scale. For    the experiments, <sup>13</sup>C-labeled polyethylene (<sup>13</sup>C-PE)    particles (irradiated with UV-light to mimic exposure of floating plasticto sunlight) were incubated in liquid medium with    <em>Rhodococcus ruber</em> as a model organism for proof of principle. The    transfer of <sup>13</sup>C from <sup>13</sup>C-PE into the gaseous and    dissolved CO<sub>2</sub> pools translated to microbially mediated    mineralization rates of up to 1.2 % yr<sup>−1</sup> of the added PE. After    incubation, we also found highly <sup>13</sup>C-enriched membrane fatty    acids of <em>R. ruber</em> including compounds involved in cellular stress    responses. We demonstrated that isotope tracer techniques are a valuable    tool to detect and quantify microbial plastic degradation.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A stable isotope assay with 13C-labeled polyethylene to investigate plastic mineralization mediated by Rhodococcus ruber","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-31 01:32:03.259629","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Microbial plastic degradation; Polyethylene biodegradation rates; Stable isotope probing; Compound specific isotope analysis; Membrane lipids; <i>Rhodococcus ruber</i>","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-02-13","DateCreate":"2023-02-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000993801900001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.marpolbul.2022.114369"},"refs":null,"anarec":{"AnaID":361114,"PubliDate":2023,"Pagination":"114369","XtraPublOfAnaID":null,"ISBN":null,"Volume":"186","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43520,"SerRR":"Marine Pollution Bulletin. 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