{"refrec":{"BRefID":361353,"RR":"<b>Bizic, M.; Brad, T.; Ionescu, D.; Barbu-Tudoran, L.; Zoccarato, L.; Aerts, J.W.; Contarini, P.-E.; Gros, O.; Volland, J.-M.; Popa, R.; Ody, J.; Vellone, D.; Flot, J.-F.; Tighe, S.; Sarbu, S.M.</b> (2023). Cave <i>Thiovulum</i> (Candidatus Thiovulum stygium) differs metabolically and genomically from marine species. <i>ISME J. 17</i>: 340-353. <a href=\"https://dx.doi.org/10.1038/s41396-022-01350-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41396-022-01350-4</a>","BEntID":359068,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>ISME J. 17</i>: 340-353. <a href=\"https://dx.doi.org/10.1038/s41396-022-01350-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41396-022-01350-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bizic, M.; Brad, T.; Ionescu, D.; Barbu-Tudoran, L.; Zoccarato, L.; Aerts, J.W.; Contarini, P.-E.; Gros, O.; Volland, J.-M.; Popa, R.; Ody, J.; Vellone, D.; Flot, J.-F.; Tighe, S.; Sarbu, S.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bizic, M. <i>et al.</i>","Englishabstract":"<i>Thiovulum</i> spp. (Campylobacterota) are large sulfur bacteria that form veil-like structures in aquatic environments. The sulfidic Movile Cave (Romania), sealed from the atmosphere for ~5 million years, has several aqueous chambers, some with low atmospheric O<sub>2</sub> (~7%). The cave’s surface-water microbial community is dominated by bacteria we identified as <i>Thiovulum</i>. We show that this strain, and others from subsurface environments, are phylogenetically distinct from marine <i>Thiovulum</i>. We assembled a closed genome of the Movile strain and confirmed its metabolism using RNAseq. We compared the genome of this strain and one we assembled from public data from the sulfidic Frasassi caves to four marine genomes, including <i>Candidatus</i> Thiovulum karukerense and <i>Ca</i>. T. imperiosus, whose genomes we sequenced. Despite great spatial and temporal separation, the genomes of the Movile and Frasassi Thiovulum were highly similar, differing greatly from the very diverse marine strains. We concluded that cave <i>Thiovulum</i> represent a new species, named here <i>Candidatus</i> Thiovulum stygium. Based on their genomes, cave <i>Thiovulum</i> can switch between aerobic and anaerobic sulfide oxidation using O<sub>2</sub> and NO<sub>3</sub><sup>-</sup> as electron acceptors, the latter likely via dissimilatory nitrate reduction to ammonia. Thus, <i>Thiovulum</i> is likely important to both S and N cycles in sulfidic caves. Electron microscopy analysis suggests that at least some of the short peritrichous structures typical of <i>Thiovulum</i> are type IV pili, for which genes were found in all strains. These pili may play a role in veil formation, by connecting adjacent cells, and in the motility of these exceptionally fast swimmers.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cave <i>Thiovulum</i> (Candidatus Thiovulum stygium) differs metabolically and genomically from marine species","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:32:29.794700","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-02-23","DateCreate":"2023-02-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000900070100001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41396-022-01350-4"},"refs":null,"anarec":{"AnaID":361353,"PubliDate":2023,"Pagination":"340-353","XtraPublOfAnaID":null,"ISBN":null,"Volume":"17","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":120798,"SerRR":"The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 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