{"refrec":{"BRefID":361548,"RR":"<b>Alsaadi, A.; Ganesen, S.S.K.; Amelia, T.S.M.; Moanis, R.; Peeters, E.; Vigneswari, S.; Bhubalan, K.</b> (2022). Polyhydroxyalkanoate (PHA) biopolymer synthesis by marine bacteria of the Malaysian coral triangle region and mining for PHA synthase genes. <i>Microorganisms 10(10)</i>: 2057. <a href=\"https://dx.doi.org/10.3390/microorganisms10102057\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms10102057</a>","BEntID":359264,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Microorganisms 10(10)</i>: 2057. <a href=\"https://dx.doi.org/10.3390/microorganisms10102057\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms10102057</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Alsaadi, A.; Ganesen, S.S.K.; Amelia, T.S.M.; Moanis, R.; Peeters, E.; Vigneswari, S.; Bhubalan, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Alsaadi, A. <i>et al.</i>","Englishabstract":"Polyhydroxyalkanoate (PHA), a biodegradable and plastic-like biopolymer, has been receiving research and industrial attention due to severe plastic pollution, resource depletion, and global waste issues. This has spurred the isolation and characterisation of novel PHA-producing strains through cultivation and non-cultivation approaches, with a particular interest in genes encoding PHA synthesis pathways. Since sea sponges and sediment are marine benthic habitats known to be rich in microbial diversity, sponge tissues (<i>Xestospongia muta</i> and <i>Aaptos aaptos</i>) and sediment samples were collected in this study from Redang and Bidong islands located in the Malaysian Coral Triangle region. PHA synthase (<i>phaC</i>) genes were identified from sediment-associated bacterial strains using a cultivation approach and from sponge-associated bacterial metagenomes using a non-cultivation approach. In addition, phylogenetic diversity profiling was performed for the sponge-associated bacterial community using 16S ribosomal ribonucleic acid (16S rRNA) amplicon sequencing to screen for the potential presence of PHA-producer taxa. A total of three <i>phaC</i> genes from the bacterial metagenome of <i>Aaptos</i> and three <i>phaC</i> genes from sediment isolates (<i>Sphingobacterium mizutaii</i> UMTKB-6, <i>Alcaligenes faecalis</i> UMTKB-7, <i>Acinetobacter calcoaceticus</i> UMTKB-8) were identified. Produced PHA polymers were shown to be composed of 5C to <i>n</i>C monomers, with previously unreported PHA-producing ability of the <i>S. mizutaii</i> strain, as well as a 3-hydroxyvalerate-synthesising ability without precursor addition by the <i>A. calcoaceticus</i> strain.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Polyhydroxyalkanoate (PHA) biopolymer synthesis by marine bacteria of the Malaysian coral triangle region and mining for PHA synthase genes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bioplastic; PHA synthase; phaC; sediment; marine sponge; diversity","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-06","DateCreate":"2023-02-27","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000875322500001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/microorganisms10102057"},"refs":null,"anarec":{"AnaID":361548,"PubliDate":2022,"Pagination":"2057","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":302866,"SerRR":"Microorganisms. 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