{"refrec":{"BRefID":362059,"RR":"<b>Situmorang, M.L.; Uawisetwathana, U.; Arayamethakorn, S.; Karoonuthaisiri, N.; Rungrassamee, W.; Haniswita, H.; Bossier, P.; Suantika, G.</b> (2022). Supplementation of ex situ produced bioflocs improves immune response against AHPND in Pacific whiteleg shrimp (<i>Litopenaeus vannamei</i>) postlarvae. <i>Appl. Microbiol. Biotechnol. 106(9-10)</i>: 3751-3764. <a href=\"https://dx.doi.org/10.1007/s00253-022-11966-3\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-022-11966-3</a>","BEntID":359775,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Microbiol. Biotechnol. 106(9-10)</i>: 3751-3764. <a href=\"https://dx.doi.org/10.1007/s00253-022-11966-3\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-022-11966-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Situmorang, M.L.; Uawisetwathana, U.; Arayamethakorn, S.; Karoonuthaisiri, N.; Rungrassamee, W.; Haniswita, H.; Bossier, P.; Suantika, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Situmorang, M.L. <i>et al.</i>","Englishabstract":"The emergence of <i>Vibrio</i> diseases, including acute hepatopancreatic necrosis disease (AHPND) caused by <i>Vibrio</i> spp., had resulted in heavy losses in global shrimp production. Biofloc technology is a closed aquaculture system developed as one of the sustainable solutions to increase system resilience in the shrimp industry. In this study, biofloc was formed externally (ex situ biofloc) with probiotics <i>Bacillus</i> sp. strain BME and <i>Bacillus</i> sp. strain BCE, diatom microalgae <i>Chaetoceros calcitrans</i>, and a consortium of nitrifying bacteria, in the ratio of 1:1:6:6 as a starter. The study showed that the ex situ biofloc supplementation in Pacific whiteleg shrimp (<i>L. vannamei</i>) postlarvae culture can increase the shrimp culture performance (shrimp survival and growth), reduce <i>Vibrio</i> counts in the water and shrimp body, and provide stimulation of the shrimp immune response through humoral immune responses, such as pattern recognition protein (<i>C-type lectin</i>) and melanization process (<i>proPO</i>). Overall, the results indicate that the supplementation of ex situ biofloc provided protection to shrimp under <i>Vibrio</i> infection, regardless of the timing of addition (before, simultaneously, or after addition of <i>Vibrio</i> sp. strain VPA). This suggests that the ex situ biofloc can be effective as a preventive and a supportive treatment against potential AHPND infection in <i>L. vannamei</i> postlarvae culture. Taken together, the ability of the ex situ biofloc to modulate immune-related gene expression and resistance of <i>L. vannamei</i> against potentially AHPND-causing <i>Vibrio</i> sp. strain makes it an effective aquaculture technology for infectious disease control in shrimp production with high-density and minimal water exchange culture.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Supplementation of ex situ produced bioflocs improves immune response against AHPND in Pacific whiteleg shrimp (<i>Litopenaeus vannamei</i>) postlarvae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-23 01:33:21.103478","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-13","DateCreate":"2023-03-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000795201000003","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00253-022-11966-3"},"refs":null,"anarec":{"AnaID":362059,"PubliDate":2022,"Pagination":"3751-3764","XtraPublOfAnaID":null,"ISBN":null,"Volume":"106","Issue":"9-10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42367,"SerRR":"Applied Microbiology and Biotechnology. 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