{"refrec":{"BRefID":362308,"RR":"<b>Moynihan, M.A.; Goodkin, N.F.; Morgan, K.M.; Kho, P.Y.Y.; dos Santos, A.L.; Lauro, F.M.; Baker, D.M.; Martin, P.</b> (2022). Coral-associated nitrogen fixation rates and diazotrophic diversity on a nutrient-replete equatorial reef. <i>ISME J. 16(1)</i>: 233-246. <a href=\"https://dx.doi.org/10.1038/s41396-021-01054-1\" target=\"_blank\">https://dx.doi.org/10.1038/s41396-021-01054-1</a>","BEntID":360024,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>ISME J. 16(1)</i>: 233-246. <a href=\"https://dx.doi.org/10.1038/s41396-021-01054-1\" target=\"_blank\">https://dx.doi.org/10.1038/s41396-021-01054-1</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Moynihan, M.A.; Goodkin, N.F.; Morgan, K.M.; Kho, P.Y.Y.; dos Santos, A.L.; Lauro, F.M.; Baker, D.M.; Martin, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Moynihan, M.A. <i>et al.</i>","Englishabstract":"The role of diazotrophs in coral physiology and reef biogeochemistry remains poorly understood, in part because N<sub>2</sub> fixation rates and diazotrophic community composition have only been jointly analyzed in the tissue of one tropical coral species. We performed field-based <sup>15</sup>N<sub>2</sub> tracer incubations during nutrient-replete conditions to measure diazotroph-derived nitrogen (DDN) assimilation into three species of scleractinian coral (<i>Pocillopora acuta</i>, <i>Goniopora columna</i>, <i>Platygyra sinensis</i>). Using multi-marker metabarcoding (16S rRNA, <i>nifH</i>, 18S rRNA), we analyzed DNA- and RNA-based communities in coral tissue and skeleton. Despite low N<sub>2</sub> fixation rates, DDN assimilation supplied up to 6% of the holobiont’s N demand. Active coral-associated diazotrophs were chiefly Cluster I (aerobes or facultative anaerobes), suggesting that oxygen may control coral-associated diazotrophy. Highest N<sub>2</sub> fixation rates were observed in the endolithic community (0.20 µg N cm<sup>−2</sup> per day). While the diazotrophic community was similar between the tissue and skeleton, RNA:DNA ratios indicate potential differences in relative diazotrophic activity between these compartments. In <i>Pocillopora</i>, DDN was found in endolithic, host, and symbiont compartments, while diazotrophic <i>nifH</i> sequences were only observed in the endolithic layer, suggesting a possible DDN exchange between the endolithic community and the overlying coral tissue. Our findings demonstrate that coral-associated diazotrophy is significant, even in nutrient-rich waters, and suggest that endolithic microbes are major contributors to coral nitrogen cycling on reefs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Coral-associated nitrogen fixation rates and diazotrophic diversity on a nutrient-replete equatorial reef","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-25 01:33:34.475901","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-20","DateCreate":"2023-03-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000675794200001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41396-021-01054-1"},"refs":null,"anarec":{"AnaID":362308,"PubliDate":2022,"Pagination":"233-246","XtraPublOfAnaID":null,"ISBN":null,"Volume":"16","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":120798,"SerRR":"The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 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