{"refrec":{"BRefID":362444,"RR":"<b>Nguyen, V.D.; Christiaens, O.; De Vos, S.; Smagghe, G.; Bossier, P.</b> (2022). The sex-specific splicing of doublesex in brine shrimp <i>Artemia franciscana</i>. <i>Genes 13(11)</i>: 1997. <a href=\"https://dx.doi.org/10.3390/genes13111997\" target=\"_blank\">https://dx.doi.org/10.3390/genes13111997</a>","BEntID":360161,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Genes 13(11)</i>: 1997. <a href=\"https://dx.doi.org/10.3390/genes13111997\" target=\"_blank\">https://dx.doi.org/10.3390/genes13111997</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nguyen, V.D.; Christiaens, O.; De Vos, S.; Smagghe, G.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nguyen, V.D. <i>et al.</i>","Englishabstract":"The understanding of sex determination and differentiation in animals has recently made remarkable strides through the use of advanced research tools. At the gene level, the Mab-3-related transcription factor (<i>Dmrt</i>) gene family, which encodes for the typical DNA-binding doublesex/Mab-3 (DM) domain in their protein, is known for its contribution to sex determination and differentiation in insects. In this study, DNA-binding DM domain screening has identified eight transcripts from <i>Artemia franciscana</i> transcriptomic that encode proteins containing one conserved DNA-binding DM domain. The genome mapping confirmed that these eight transcripts are transcribed from six different loci on the <i>A. franciscana</i> genome assembly. One of those loci, the Af.dsx-4 locus, is closely related to <i>Doublesex</i>, a gene belonging to the <i>Dmrt</i> gene family. This locus could be transcribed into three alternative transcripts, namely Af.dsx<sup>4</sup>, Af.dsx<sup>F</sup> and Af.dsx<sup>M</sup>. While Af.dsx<sup>4</sup> and Af.dsx<sup>F</sup> could putatively be translated to form an identical Af.dsx<sup>F</sup> protein of 186 aa long, Af.dsx<sup>M</sup> translates for an Af.dsx<sup>M</sup> protein of 289 aa long but shares a DNA-binding DM domain. Interestingly, Af.dsx<sup>F</sup> and Af.dsx<sup>M</sup> are confirmed as sex-specific transcripts, Af.dsx<sup>F</sup> is only present in females, and Af.dsx<sup>M</sup> is only present in male individuals. The results suggest that the sex-specific splicing mechanism of the <i>doublesex</i> described in insects is also present in <i>A. franciscana</i>. Af.dxs-4 locus can be used in further studies to clarify the sex determination pathways in <i>A. fracnciscana</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The sex-specific splicing of doublesex in brine shrimp <i>Artemia franciscana</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Artemia franciscana; Doublesex; sex-specific splicing","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-27","DateCreate":"2023-03-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000881187200001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/genes13111997"},"refs":null,"anarec":{"AnaID":362444,"PubliDate":2022,"Pagination":"1997","XtraPublOfAnaID":null,"ISBN":null,"Volume":"13","Issue":"11","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":268585,"SerRR":"Genes. 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