{"refrec":{"BRefID":362446,"RR":"<b>Kalina, R.S.; Kasheverov, I.E.; Koshelev, S.G.; Sintsova, O.V.; Peigneur, S.; Pinheiro-Junior, E.L.; Popov, R.S.; Chausova, V.; Monastyrnaya, M.M.; Dmitrenok, P.S.; Isaeva, M.P.; Tytgat, J.; Kozlov, S.A.; Kozlovskaya, E.P.; Leychenko, E.V.; Gladkikh, I.N.</b> (2022). Nicotinic acetylcholine receptors are novel targets of APETx-like toxins from the sea anemone <i>Heteractis magnifica</i>. <i>Toxins 14(10)</i>: 697. <a href=\"https://dx.doi.org/10.3390/toxins14100697\" target=\"_blank\">https://dx.doi.org/10.3390/toxins14100697</a>","BEntID":360163,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Toxins 14(10)</i>: 697. <a href=\"https://dx.doi.org/10.3390/toxins14100697\" target=\"_blank\">https://dx.doi.org/10.3390/toxins14100697</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kalina, R.S.; Kasheverov, I.E.; Koshelev, S.G.; Sintsova, O.V.; Peigneur, S.; Pinheiro-Junior, E.L.; Popov, R.S.; Chausova, V.; Monastyrnaya, M.M.; Dmitrenok, P.S.; Isaeva, M.P.; Tytgat, J.; Kozlov, S.A.; Kozlovskaya, E.P.; Leychenko, E.V.; Gladkikh, I.N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kalina, R.S. <i>et al.</i>","Englishabstract":"The nicotinic acetylcholine receptors (nAChRs) are prototypical ligand-gated ion channels, provide cholinergic signaling, and are modulated by various venom toxins and drugs in addition to neurotransmitters. Here, four APETx-like toxins, including two new toxins, named Hmg 1b-2 Met<sub>ox</sub> and Hmg 1b-5, were isolated from the sea anemone <i>Heteractis magnifica</i> and characterized as novel nAChR ligands and acid-sensing ion channel (ASIC) modulators. All peptides competed with radiolabeled α-bungarotoxin for binding to <i>Torpedo californica</i> muscle-type and human α7 nAChRs. Hmg 1b-2 potentiated acetylcholine-elicited current in human α7 receptors expressed in <i>Xenopus laevis</i> oocytes. Moreover, the multigene family coding APETx-like peptides library from <i>H. magnifica</i> was described and in silico surface electrostatic potentials of novel peptides were analyzed. To explain the 100% identity of some peptide isoforms between <i>H. magnifica</i> and <i>H. crispa</i>, 18S rRNA, COI, and ITS analysis were performed. It has been shown that the sea anemones previously identified by morphology as <i>H. crispa</i> belong to the species <i>H. magnifica</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Nicotinic acetylcholine receptors are novel targets of APETx-like toxins from the sea anemone <i>Heteractis magnifica</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"sea anemones; phylogeny; APETx-like toxins; nicotinic acetylcholine receptors; acid-sensing ion channels","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-22","DateCreate":"2023-03-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000875284500001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/toxins14100697"},"refs":null,"anarec":{"AnaID":362446,"PubliDate":2022,"Pagination":"697","XtraPublOfAnaID":null,"ISBN":null,"Volume":"14","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":197331,"SerRR":"Toxins. 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