{"refrec":{"BRefID":362450,"RR":"Zheng, L.; Byadgi, O.; Rakhshaninejad, M.; Nauwynck, H. (2022). Upregulation of torso-like protein (perforin) and granzymes B and G in non-adherent, lymphocyte-like haemocytes during a WSSV infection in shrimp. Fish Shellfish Immunol. 128: 676-683. https://dx.doi.org/10.1016/j.fsi.2022.08.021","BEntID":360167,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". Fish Shellfish Immunol. 128: 676-683. https://dx.doi.org/10.1016/j.fsi.2022.08.021","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zheng, L.; Byadgi, O.; Rakhshaninejad, M.; Nauwynck, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zheng, L. et al.","Englishabstract":"Invertebrates only have an innate immunity in which haemocytes play an important role. In our lab, 5 subpopulations of haemocytes were identified in the past by an iodixanol density gradient: hyalinocytes, granulocytes, semi-granulocytes and two subpopulations of non-phagocytic cells. For the two latter subpopulations, the haemocytes have small cytoplasm rims, do not adhere to the bottom of plastic cell-culture grade wells and present folds in the nucleus. These characteristics are similar to those of mammalian lymphocytes. Therefore, they were designated lymphocyte-like haemocytes. Although little is known about their function, we hypothesize, based on their morphology, that they may have a cytotoxic activity. First, a fast isolation technique was developed to separate the non-adherent haemocytes from the adherent haemocytes. After 60 min incubation on cell culture plates, the non-adherent haemocytes were collected. The purity reached 93% as demonstrated by flow cytometry and light microscopy upon a Hematoxylin and Eosin (H&E) staining. Cytotoxicity by lymphocytes is mediated by molecules such as perforin and granzymes and therefore, we searched for their genes in the shrimp genome. Genes coding for a torso-like protein, granzyme B and granzyme G were identified. Primers were designed and RT-PCR/RT-qPCR assays were developed. The results demonstrated that torso-like protein, granzyme B and granzyme G were mainly expressed in non-adherent haemocytes. The shrimp torso-like protein gene was most related to that of the crab torso-like protein; granzyme B gene was most related to that of mouse granzyme B and granzyme G gene was most related to that of zebrafish granzyme G. In a 72-hour in vivo WSSV infection challenge, the mRNA expression of shrimp torso-like protein, granzyme B and granzyme G in haemocytes was increasing over time, which indicated that torso-like protein, granzyme B and granzyme G of shrimp haemocytes are involved in the immune response during a viral infection. In the future, antibodies will be raised against these proteins for more in-depth functional analyses.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Upregulation of torso-like protein (perforin) and granzymes B and G in non-adherent, lymphocyte-like haemocytes during a WSSV infection in shrimp","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:48.823240","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-03-27","DateCreate":"2023-03-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000919363100003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.fsi.2022.08.021"},"refs":null,"anarec":{"AnaID":362450,"PubliDate":2022,"Pagination":"676-683","XtraPublOfAnaID":null,"ISBN":null,"Volume":"128","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45027,"SerRR":"Fish & Shellfish Immunology. Academic Press: London; New York. ISSN 1050-4648; e-ISSN 1095-9947","StandardTitleSer":"Fish & Shellfish Immunology","ISSN":"1050-4648","AbbrevSer":"Fish Shellfish Immunol.","StandardTitleMon":null,"StartPage":676,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Zheng","Firstname":"Liping","Initials":"L.","Affiliation":"Laboratory of Virology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":360167,"AutID":522398,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Diergeneeskunde; Vakgroep Virologie, Parasitologie en Immunologie; Laboratory of Virology","ORCID":"0000-0001-5604-6890","PersID":42435,"InsID":13137},{"AutName":"Byadgi","Firstname":"Omkar","Initials":"O.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":360167,"AutID":522399,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Rakhshaninejad","Firstname":"Mostafa","Initials":"M.","Affiliation":"Laboratory of Virology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":360167,"AutID":522400,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Diergeneeskunde; Vakgroep Virologie, Parasitologie en Immunologie; Laboratory of Virology","ORCID":null,"PersID":42436,"InsID":13137},{"AutName":"Nauwynck","Firstname":"Hans","Initials":"H.","Affiliation":"Laboratory of Virology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":360167,"AutID":522401,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Diergeneeskunde; Vakgroep Virologie, Parasitologie en Immunologie; Laboratory of Virology","ORCID":"0000-0001-5470-0713","PersID":26225,"InsID":13137}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":388230,"LibID":36,"BRefID":362450,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"388230","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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