{"refrec":{"BRefID":362777,"RR":"<b>Barbato, M.; Scoma, A.</b> (2020). Mild hydrostatic-pressure (15 MPa) affects the assembly, but not the growth, of oil-degrading coastal microbial communities tested under limiting conditions (5°C, no added nutrients). <i>FEMS Microbiol. Ecol. 96(11)</i>: fiaa160. <a href=\"https://dx.doi.org/10.1093/femsec/fiaa160\" target=\"_blank\">https://dx.doi.org/10.1093/femsec/fiaa160</a>","BEntID":360495,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>FEMS Microbiol. Ecol. 96(11)</i>: fiaa160. <a href=\"https://dx.doi.org/10.1093/femsec/fiaa160\" target=\"_blank\">https://dx.doi.org/10.1093/femsec/fiaa160</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Barbato, M.; Scoma, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Barbato, M.; Scoma, A.","Englishabstract":"Hydrostatic pressures (HP) &lt;30–40&nbsp;MPa&nbsp;are often considered mild, and their impact on petroleum biodegradation seldom considered. However, the frequent use of nutrient-rich media in lab-scale high-pressure reactors may exaggerate HP importance by resulting in a strong growth stimulation as compared to oligotrophic marine environments. Here, we tested coastal seawater microbial communities, presumably enriched in pressure-sensitive microorganisms. Limiting environmental conditions for growth were applied (i.e. low temperature [5°C], no added nutrients) and HP tested at 0.1 and 15&nbsp;MPa, using crude oils from three different reservoirs. The cell number was not affected by HP contrary to the microbial community composition (based on 16S rRNA gene and 16S rRNA sequences). The most predominant genera were <em>Zhongshania</em>, <em>Pseudomonas</em> and <em>Colwellia</em>. The enrichment of <em>Zhongshania</em> was crude-oil dependent and comparable at 0.1 and 15&nbsp;MPa, thus showing a piezotolerant phenotype under the present conditions; <em>Pseudomonas’</em> was crude-oil dependent at 0.1&nbsp;MPa but unclear at 15&nbsp;MPa. <em>Colwellia</em> was selectively enriched in the absence of crude oil and suppressed at 15&nbsp;MPa. HP shaped the assemblage of oil-degrading communities even at mild levels (i.e. 15&nbsp;MPa), and should thus be considered as a fundamental factor to assess oil bioremediation along the water column.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Mild hydrostatic-pressure (15 MPa) affects the assembly, but not the growth, of oil-degrading coastal microbial communities tested under limiting conditions (5°C, no added nutrients)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-30 01:32:03.958501","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"hydrostatic pressure, petroleum hydrocarbon, amplicon sequencing, flow cytometry, oil degradation, bioremediation","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2023-03-29","DateCreate":"2023-03-27","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000593018400004","VABBcode":null,"OpenAcc":0,"DOI":"10.1093/femsec/fiaa160"},"refs":null,"anarec":{"AnaID":362777,"PubliDate":2020,"Pagination":"fiaa160","XtraPublOfAnaID":null,"ISBN":null,"Volume":"96","Issue":"11","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42870,"SerRR":"FEMS Microbiology Ecology. Federation of European Microbiological Societies: Amsterdam.  ISSN 0168-6496; e-ISSN 1574-6941","StandardTitleSer":"FEMS Microbiology Ecology","ISSN":"0168-6496","AbbrevSer":"FEMS Microbiol. 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