{"refrec":{"BRefID":363091,"RR":"<b>Ahmed, H.A.; Ticar, B.F.; Black, I.; Mahdi, F.; Shami, A.A.; Misra, S.K.; Heiss, C.; Paris, J.J.; Sharp, J.S.; Azadi, P.; Pomin, V.H.</b> (2023). Structural characterization and biological activity of an α-glucan from the mollusk <i>Marcia hiantina</i> (Lamarck, 1818). <i>Glycoconjugate Journal 40</i>: 33-46. <a href=\"https://dx.doi.org/10.1007/s10719-022-10092-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10719-022-10092-6</a>","BEntID":360809,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Glycoconjugate Journal 40</i>: 33-46. <a href=\"https://dx.doi.org/10.1007/s10719-022-10092-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10719-022-10092-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ahmed, H.A.; Ticar, B.F.; Black, I.; Mahdi, F.; Shami, A.A.; Misra, S.K.; Heiss, C.; Paris, J.J.; Sharp, J.S.; Azadi, P.; Pomin, V.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ahmed, H.A. <i>et al.</i>","Englishabstract":"<i>Marcia hiantina</i> (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from <i>M. hiantina</i> by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D <sup>1</sup>H, <sup>1</sup>H-<sup>1</sup>H COSY, <sup>1</sup>H-<sup>1</sup>H TOCSY, <sup>1</sup>H-<sup>1</sup>H NOESY, <sup>1</sup>H-<sup>13</sup>C HSQC and <sup>1</sup>H-<sup>13</sup>C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of <i>M. hiantina</i>-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H<sub>2</sub>O<sub>2</sub>- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H<sub>2</sub>O<sub>2</sub>- and LPS-induced ROS, and potent cell proliferative activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Structural characterization and biological activity of an α-glucan from the mollusk <i>Marcia hiantina</i> (Lamarck, 1818)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bivalve; glucan; mollusk; Marcia hiantina; methylation analysis; NMR","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-05-22","DateCreate":"2023-04-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000912586400001","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10719-022-10092-6"},"refs":null,"anarec":{"AnaID":363091,"PubliDate":2023,"Pagination":"33-46","XtraPublOfAnaID":null,"ISBN":null,"Volume":"40","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":268803,"SerRR":"Glycoconjugate Journal. 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