Document of bibliographic reference 363091
BibliographicReference record
- Type
- Bibliographic resource
- Type of document
- Journal article
- BibLvlCode
- AS
- Title
- Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818)
- Abstract
- Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D 1H, 1H-1H COSY, 1H-1H TOCSY, 1H-1H NOESY, 1H-13C HSQC and 1H-13C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H2O2- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H2O2- and LPS-induced ROS, and potent cell proliferative activity.
- WebOfScience code
- https://www.webofscience.com/wos/woscc/full-record/WOS:000912586400001
- Bibliographic citation
- Ahmed, H.A.; Ticar, B.F.; Black, I.; Mahdi, F.; Shami, A.A.; Misra, S.K.; Heiss, C.; Paris, J.J.; Sharp, J.S.; Azadi, P.; Pomin, V.H. (2023). Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818). Glycoconjugate Journal 40: 33-46. https://dx.doi.org/10.1007/s10719-022-10092-6
- Topic
- Marine
- Is peer reviewed
- true
Authors
- author
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- Name
- Hoda Ahmed
- author
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- Name
- Bernadeth Ticar
- author
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- Name
- Ian Black
- author
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- Name
- Fakhri Mahdi
- author
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- Name
- Anter Shami
- author
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- Name
- Sandeep Misra
- author
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- Name
- Christian Heiss
- author
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- Name
- Jason Paris
- author
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- Name
- Joshua Sharp
- author
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- Name
- Parastoo Azadi
- author
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- Name
- Vitor Pomin