{"refrec":{"BRefID":363254,"RR":"<b>Elfeky, H.H.; Hanora, A.; Solyman, S.M.</b> (2023). Bioactivity of bacteria associated with Red Sea nudibranchs and whole genome sequence of <i>Nocardiopsis dassonvillei</i> RACA-4. <i>Marine Genomics 67</i>: 101004. <a href=\"https://dx.doi.org/10.1016/j.margen.2022.101004\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2022.101004</a>","BEntID":360972,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Marine Genomics 67</i>: 101004. <a href=\"https://dx.doi.org/10.1016/j.margen.2022.101004\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2022.101004</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Elfeky, H.H.; Hanora, A.; Solyman, S.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Elfeky, H.H.; Hanora, A.; Solyman, S.M.","Englishabstract":"Microorganisms associated with marine invertebrates consider an important source of bioactive products. This study aimed to screen for antimicrobial and anticancer activity of crude extracts of bacteria associated with Red sea nudibranchs and molecular identification of the bioactive isolates using 16Sr RNA sequencing, in addition to whole-genome sequencing of one of the most bioactive bacteria. This study showed that bacteria associated with Red sea nudibranchs are highly bioactive and 16Sr RNA sequencing results showed that two isolates belonged to Firmicutes, and two isolates belonged to Proteobacteria, and Actinobacteria. The whole genome sequence data of the isolated <i>Nocardiopsis RACA4</i> isolate has an estimated genome length of 6,721,839 bp and the taxonomy showed it belongs to the bacteria <i>Nocardiopsis dassonvillei</i>. The De novo assembly of RACA-4 paired reads using Unicycler v0.4.8 initially yielded 97 contigs with an N50 value of 214,568 bp and L50 value of 10, The resulting assembly was further mapped to the reference genome <i>Nocardiopsis dassonvillei</i> strain NCTC10488 using RagTag software v.2.1.0 and a final genome assembly resulted in 39 contigs and N50 value of 6,726,007 and L50 of 1. Genome mining using anti-smash showed around 9.1% of the genome occupied with genes related to secondary metabolites biosynthesis. A wide variety of secondary metabolites belonging to Polyketides, Terpenes, and nonribosomal peptides were predicted with high degree of similarity to known compounds. Non-characterized clusters were also found which suggest new natural compounds discovered by further studies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Bioactivity of bacteria associated with Red Sea nudibranchs and whole genome sequence of <i>Nocardiopsis dassonvillei</i> RACA-4","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Red Sea nudibranchs; antimicrobial; antitumor; secondary metabolites whole genome sequence","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-05-23","DateCreate":"2023-04-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000903969200001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.margen.2022.101004"},"refs":null,"anarec":{"AnaID":363254,"PubliDate":2023,"Pagination":"101004","XtraPublOfAnaID":null,"ISBN":null,"Volume":"67","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":126432,"SerRR":"Marine Genomics. 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