{"refrec":{"BRefID":363255,"RR":"<b>Gaonkar, C.C.; Campbell, L.</b> (2023). Metabarcoding reveals high genetic diversity of harmful algae in the coastal waters of Texas, Gulf of Mexico. <i>Harmful Algae 121</i>: 102368. <a href=\"https://dx.doi.org/10.1016/j.hal.2022.102368\" target=\"_blank\">https://dx.doi.org/10.1016/j.hal.2022.102368</a>","BEntID":360973,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Harmful Algae 121</i>: 102368. <a href=\"https://dx.doi.org/10.1016/j.hal.2022.102368\" target=\"_blank\">https://dx.doi.org/10.1016/j.hal.2022.102368</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Gaonkar, C.C.; Campbell, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gaonkar, C.C.; Campbell, L.","Englishabstract":"Environmental-DNA (eDNA) for metabarcoding is a rapid and effective means to investigate microplankton community composition and species diversity. The objective of this study was to examine the genetic diversity of the phytoplankton community in the Gulf of Mexico, with particular emphasis on harmful algal bloom species. Samples were collected at stations along the coast of Texas in September-October 2017 that were inundated by low salinity waters in the aftermath of Hurricane Harvey. Metabarcodes were generated from the eDNA targeting both the V4 and V8-V9 regions of the 18S rDNA gene. Evaluation of the metabarcodes revealed an unexpectedly high number of harmful algal species during this short period, including five that had not been documented in this region previously. A total of 36 harmful algal species could be differentiated based on V4 and V8-V9 metabarcode markers. Using a phylogenetic approach, the taxonomic resolution of each marker differed and not all species could be differentiated using solely one marker. The V4 region resolved species within some genera (e.g., <i>Heterocapsa</i>), while the V8-V9 marker was necessary to resolve species within other genera (e.g., Chattonella). In other cases, species differentiation within a genus required a combination of both markers (e.g., Prorocentrum, Karenia), or another marker will be needed to resolve all species (e.g., Alexandrium, Dinophysis). We conclude that no single marker can delineate all species, so it is recommended HAB monitoring programs use more than one marker. 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