{"refrec":{"BRefID":363432,"RR":"<b>Lavrador, A.S.; Fontes, J.T.; Vieira, P.E.; Costa, F.O.; Duarte, S.</b> (2023). Compilation, revision, and annotation of DNA barcodes of marine invertebrate non-indigenous species (NIS) occurring in European coastal regions. <i>Diversity 15(2)</i>: 174. <a href=\"https://dx.doi.org/10.3390/d15020174\" target=\"_blank\">https://dx.doi.org/10.3390/d15020174</a>","BEntID":361150,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Diversity 15(2)</i>: 174. <a href=\"https://dx.doi.org/10.3390/d15020174\" target=\"_blank\">https://dx.doi.org/10.3390/d15020174</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lavrador, A.S.; Fontes, J.T.; Vieira, P.E.; Costa, F.O.; Duarte, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lavrador, A.S. <i>et al.</i>","Englishabstract":"<div class=\"html-p\">The introduction of non-indigenous species (NIS) is one of the major threats to the integrity of European coastal ecosystems. DNA-based assessments have been increasingly adopted for monitoring NIS. However, the accuracy of DNA-based taxonomic assignments is largely dependent on the completion and reliability of DNA barcode reference libraries. As such, we aimed to compile and audit a DNA barcode reference library for marine invertebrate NIS occurring in Europe. To do so, we compiled a list of NIS using three databases: the European Alien Species Information Network (EASIN), the Information System on Aquatic Non-indigenous and Cryptogenic Species (AquaNIS), and the World Register of Introduced Marine Species (WRiMS). For each species, we retrieved the available cytochrome c oxidase subunit I (COI) mitochondrial gene sequences from the Barcode of Life Data System (BOLD) and used the Barcode, Audit & Grade System (BAGS) to check congruence between morphospecies names and Barcode Index Numbers (BINs). From the 1249 species compiled, approximately 42% had records on BOLD, among which 56% were discordant. We further analyzed these cases to determine the causes of the discordances and attributed additional annotation tags. Of the 622 discordant BINs, after revision, 35% were successfully solved, which increased the number of NIS detected in metabarcoding datasets from 12 to 16. However, a fair number of BINs remained discordant. Reliability of reference barcode records is particularly critical in the case of NIS, where erroneous identification may trigger action or inaction when not required.</div>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Compilation, revision, and annotation of DNA barcodes of marine invertebrate non-indigenous species (NIS) occurring in European coastal regions","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"European coastal ecosystems; DNA reference libraries; COI barcode sequences","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-04-11","DateCreate":"2023-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000939035600001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/d15020174"},"refs":null,"anarec":{"AnaID":363432,"PubliDate":2023,"Pagination":"174","XtraPublOfAnaID":null,"ISBN":null,"Volume":"15","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":239468,"SerRR":"Diversity. 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