{"refrec":{"BRefID":363668,"RR":"<b>Van den Bulcke, L.; De Backer, A.; Wittoeck, J.; Beentjes, K.; Maes, S.; Christodoulou, M.; Martinez Arbizu, P.; Sapkota, R.; van der Hoorn, B.; Winding, A.; Hostens, K.; Derycke, S.</b> (2023). DNA metabarcoding on repeat: sequencing data of marine macrobenthos are reproducible and robust across labs and protocols. <i>Ecol. Indic. 150</i>: 110207. <a href=\"https://dx.doi.org/10.1016/j.ecolind.2023.110207\" target=\"_blank\">https://dx.doi.org/10.1016/j.ecolind.2023.110207</a>","BEntID":361387,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Ecol. Indic. 150</i>: 110207. <a href=\"https://dx.doi.org/10.1016/j.ecolind.2023.110207\" target=\"_blank\">https://dx.doi.org/10.1016/j.ecolind.2023.110207</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Van den Bulcke, L.; De Backer, A.; Wittoeck, J.; Beentjes, K.; Maes, S.; Christodoulou, M.; Martinez Arbizu, P.; Sapkota, R.; van der Hoorn, B.; Winding, A.; Hostens, K.; Derycke, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Van den Bulcke, L. <i>et al.</i>","Englishabstract":"DNA metabarcoding can be used in marine environmental monitoring if results are reproducible between labs and robust against modifications to the lab protocol. In this interlaboratory study, we conducted a ring test where subsamples of blended macrobenthos samples were distributed to four laboratories located in Belgium, the Netherlands, Germany and Denmark. Samples were processed by a standardized lab protocol and by an adapted protocol, and the resulting datasets were analyzed with the same bioinformatics pipeline. Different biodiversity indicators were calculated. Our results show that bulkDNA metabarcoding of marine macrobenthos offers a highly reproducible assessment of alpha diversity patterns when using a standardized protocol, since comparable species numbers, Shannon indices and Inverse Simpson indices were found between laboratories. Especially high abundant species and species with large body sizes where shared between the laboratories. The need for using a standardized protocol to enhance comparability in alpha diversity between different studies was shown. Beta diversity patterns are less subjected to changes in the metabarcoding protocol and were almost identical between different laboratories, as the main clustering was always based on the macrobenthic community, independent of the used protocol or the laboratory that conducted the work. We conclude that DNA metabarcoding for marine environmental monitoring is an appropriate method when the aim is to study changes in community patterns and advocate its implementation in routine monitoring programs of national and European authorities, providing that a standardized protocol is implemented and/or a detailed description of the protocol is available.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"DNA metabarcoding on repeat: sequencing data of marine macrobenthos are reproducible and robust across labs and protocols","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:33:17.271674","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Marine environmental monitoring; North Sea; COI; Standardized operational protocol; Ring test; Standard operating procedures (SOPS)","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2023-04-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000982578500001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.ecolind.2023.110207"},"refs":null,"anarec":{"AnaID":363668,"PubliDate":2023,"Pagination":"110207","XtraPublOfAnaID":null,"ISBN":null,"Volume":"150","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45477,"SerRR":"Ecological Indicators. Elsevier: Shannon.  ISSN 1470-160X; e-ISSN 1872-7034","StandardTitleSer":"Ecological Indicators","ISSN":"1470-160X","AbbrevSer":"Ecol. Indic.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Van den Bulcke","Firstname":"Laure","Initials":"L.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529051,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":null,"PersID":35434,"InsID":5442},{"AutName":"De Backer","Firstname":"Annelies","Initials":"A.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529052,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":"0000-0001-9129-9009","PersID":3466,"InsID":5442},{"AutName":"Wittoeck","Firstname":"Jan","Initials":"J.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529053,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":null,"PersID":715,"InsID":5442},{"AutName":"Beentjes","Firstname":"Kevin","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529054,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Maes","Firstname":"Sara","Initials":"S.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529055,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":null,"PersID":26702,"InsID":5442},{"AutName":"Christodoulou","Firstname":"Magdalini","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":52725,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Martinez Arbizu","Firstname":"Pedro","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":16733,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-0891-1154","PersID":7550,"InsID":null},{"AutName":"Sapkota","Firstname":"Rumakanta","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":458044,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"van der Hoorn","Firstname":"Berry","Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":361387,"AutID":91032,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Winding","Firstname":"Anne","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":477975,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hostens","Firstname":"Kris","Initials":"K.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529061,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":"0000-0002-8476-1362","PersID":226,"InsID":5442},{"AutName":"Derycke","Firstname":"Sofie","Initials":"S.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food – Marine Research","Discriminator":null,"CorporateFlag":0,"BEntID":361387,"AutID":529062,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ILVO","InsFSN":"Instituut voor Landbouw-, Visserij- en Voedingsonderzoek","ORCID":"0000-0003-3763-6187","PersID":5433,"InsID":5442}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":389212,"LibID":36,"BRefID":363668,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"389212","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Shannon"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"},{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":[{"ProID":4928,"Acronym":"GEANS","ProStandardTitle":"Genetic Tools for Ecosystem Health Assessment in the North Sea Region","BYear":2019,"BMonth":3,"EYear":2023,"EMonth":6,"FP7Code":null,"RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"927":{"SpName":"Marine Data Archive (MDA) acknowledged","SpColID":927,"ParSpColID":39,"TopParID":39,"ShortName":"MDA Ack","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/MDA Ack"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"},"422":{"SpName":"Section Marine Biology","SpColID":422,"ParSpColID":null,"TopParID":null,"ShortName":"MarBiol","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"MarBiol"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":361387,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":361387,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.ecolind.2023.110207","externalID":"10.1016/j.ecolind.2023.110207","URLTypeCode":"DOI","URLID":115501,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2023-04-14 08:39:32.850000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2025-09-18 16:33:04.040000","timezone_type":3,"timezone":"Europe/Brussels"}}}