{"refrec":{"BRefID":364339,"RR":"<b>Fantino, J.-R.; Barras, F.; Denizot, F.</b> (2009). Sposensor: A whole-bacterial biosensor that uses immobilized <i>Bacillus subtilis</i> spores and a one-step incubation/detection process. <i>J. Mol. Microbiol. Biotechnol. 17(2)</i>: 90-95. <a href=\"https://dx.doi.org/10.1159/000206634\" target=\"_blank\">https://dx.doi.org/10.1159/000206634</a>","BEntID":362059,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>J. Mol. Microbiol. Biotechnol. 17(2)</i>: 90-95. <a href=\"https://dx.doi.org/10.1159/000206634\" target=\"_blank\">https://dx.doi.org/10.1159/000206634</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fantino, J.-R.; Barras, F.; Denizot, F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fantino, J.-R.; Barras, F.; Denizot, F.","Englishabstract":"A generic whole-cell bacterial sensor called sposensor was developed with immobilized spores from engineered Bacillus subtilis. Sposensor contains two different types of spores: reporting spores that contain a reporter gene fused to a promoter responding to a compound to be detected, and control spores use to monitor cell germination and viability. A one-step incubation/detection process was developed to meet the constraints of on-site analysis. Spores were directly incubated with culture medium containing the compound to be detected. beta-Galactosidase was chosen as a reporter protein in both cases and its activity followed by a colorimetric assay. Results showed that sposensor was efficient in detecting two different compounds, a metal (Zn(2+)) and a peptidic antibiotic (bacitracin). Owing to the stability and robustness of spores, sposensor is a very efficient and easy tool to manipulate for analyzing the presence of toxic compounds in natural settings.    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