{"refrec":{"BRefID":364344,"RR":"<b>Gabrielson, J.; Hart, M.; Jarelöv, A.; Kühn, I.; McKenzie, D.; Möllby, R.</b> (2002). Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates. <i>J. microbiol. methods 50(1)</i>: 63-73. <a href=\"https://dx.doi.org/10.1016/s0167-7012(02)00011-8\" target=\"_blank\">https://dx.doi.org/10.1016/s0167-7012(02)00011-8</a>","BEntID":362064,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. microbiol. methods 50(1)</i>: 63-73. <a href=\"https://dx.doi.org/10.1016/s0167-7012(02)00011-8\" target=\"_blank\">https://dx.doi.org/10.1016/s0167-7012(02)00011-8</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Gabrielson, J.; Hart, M.; Jarelöv, A.; Kühn, I.; McKenzie, D.; Möllby, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gabrielson, J. <i>et al.</i>","Englishabstract":"The growth indicators 2,3,5-triphenyltetrazolium chloride (TTC), 2-[4-iodophenyl]-3-[4-dinitrophenyl]-5-phenyltetrazolium chloride (INT), 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), and resazurin were tested for their ability to indicate bacterial growth/growth inhibition. Two reading devices were evaluated and compared, a microplate spectrophotometer and a digital flatbed scanner. The bacteria used in the study were cultivated in 96-wells microplates and readings were made after 24 h. The scanned pictures were analysed with a software developed in-house to generate numerical values. It was found that resazurin was difficult to use since it shifts between three colours. MTT and TTC had a high correlation between the spectrophotometer data and the data from the scanned images. The reproducibility was similar for both reading devices. In no case was there a need to resuspend the pellets before reading. Both the XTT and INT showed lower correlations. It is concluded that bacterial growth/growth inhibition can be easily and reproducibly measured from microplate cultivations with a flatbed scanner or with a microplate spectrophotometer.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:32:00.247598","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2023-05-15","DateCreate":"2023-05-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000175321900007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/s0167-7012(02)00011-8"},"refs":null,"anarec":{"AnaID":364344,"PubliDate":2002,"Pagination":"63-73","XtraPublOfAnaID":null,"ISBN":null,"Volume":"50","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":121411,"SerRR":"Journal of Microbiological Methods. 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