{"refrec":{"BRefID":364378,"RR":"<b>Käkinen, A.; Bondarenko, O.; Ivask, A.; Kahru, A.</b> (2011). The Effect of Composition of Different Ecotoxicological Test Media on Free and Bioavailable Copper from CuSO4 and CuO Nanoparticles: Comparative Evidence from a Cu-Selective Electrode and a Cu-Biosensor. <i>Sensors 11(11)</i>: 10502-10521. <a href=\"https://dx.doi.org/10.3390/s111110502\" target=\"_blank\">https://dx.doi.org/10.3390/s111110502</a>","BEntID":362098,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Sensors 11(11)</i>: 10502-10521. <a href=\"https://dx.doi.org/10.3390/s111110502\" target=\"_blank\">https://dx.doi.org/10.3390/s111110502</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Käkinen, A.; Bondarenko, O.; Ivask, A.; Kahru, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Käkinen, A. <i>et al.</i>","Englishabstract":"The analysis of (bio)available copper in complex environmental settings, including biological test media, is a challenging task. In this study, we demonstrated the potential of a recombinant <span class=\"html-italic\">Pseudomonas fluorescens</span>-based biosensor for bioavailability analysis of CuSO<sub>4</sub> and CuO nanoparticles (nano-CuO) in seventeen different ecotoxicological and microbiologial test media. In parallel, free Cu in these test media was analysed using Cu-ion selective electrode (Cu-ISE). In the case of CuSO<sub>4</sub>, both free and bioavailable Cu decreased greatly with increasing concentration of organics and phosphates in the tested media. A good correlation between free and bioavailable Cu was observed (r = 0.854, p &lt; 0.01) indicating that the free Cu content in biological test media may be a reasonably good predictor for the toxicity of CuSO<sub>4</sub>. As a proof, it was demonstrated that when eleven EC<sub>50</sub> values for CuSO<sub>4</sub> from different organisms in different test media were normalized for the free Cu in these media, the difference in these EC<sub>50</sub> values was decreased from 4 to 1.8 orders of magnitude. Thus, toxicity of CuSO<sub>4</sub> to these organisms was attributed to the properties of the test media rather than to inherent differences in sensitivity between the test organisms. Differently from CuSO<sub>4</sub>, the amount of free and bioavailable Cu in nano-CuO spiked media was not significantly correlated with the concentration of organics in the test media. Thus, the speciation of nano-CuO in toxicological test systems was not only determined by the complexation of Cu ions but also by differential dissolution of nano-CuO in different test conditions leading to a new speciation equilibrium. In addition, a substantial fraction of nano-CuO that was not detectable by Cu-ISE (<span class=\"html-italic\">i.e</span>., not present as free Cu-ions) was bioavailable to Cu-biosensor bacteria. Thus, in environmental hazard analysis of (nano) particulate materials, biosensor analysis may be more informative than other analytical techniques. Our results demonstrate that bacterial Cu-biosensors either in combination with other analytical/speciation techniques or on their own, may serve as a rapid (eco)toxicological screening method.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The Effect of Composition of Different Ecotoxicological Test Media on Free and Bioavailable Copper from CuSO4 and CuO Nanoparticles: Comparative Evidence from a Cu-Selective Electrode and a Cu-Biosensor","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2011,"MonPub":null,"DateUpdate":"2023-05-16","DateCreate":"2023-05-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000297698600030","VABBcode":null,"OpenAcc":0,"DOI":"10.3390/s111110502"},"refs":null,"anarec":{"AnaID":364378,"PubliDate":2011,"Pagination":"10502-10521","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"11","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":240560,"SerRR":"Sensors. 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