{"refrec":{"BRefID":364435,"RR":"<b>Kahru, A.; Drobne, D.; Singh, S.; Kalcíková, G.; Kühnel, D.; Rohit, R.; Gotvajn, A.Ž.; Jemec, A.</b> (2016). A case study to optimise and validate the brine shrimp Artemia franciscana immobilisation assay with silver nanoparticles: The role of harmonisation. <i>Environ. Pollut. 213</i>: 173-183. <a href=\"https://dx.doi.org/10.1016/j.envpol.2016.02.015\" target=\"_blank\">https://dx.doi.org/10.1016/j.envpol.2016.02.015</a>","BEntID":362155,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Pollut. 213</i>: 173-183. <a href=\"https://dx.doi.org/10.1016/j.envpol.2016.02.015\" target=\"_blank\">https://dx.doi.org/10.1016/j.envpol.2016.02.015</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kahru, A.; Drobne, D.; Singh, S.; Kalcíková, G.; Kühnel, D.; Rohit, R.; Gotvajn, A.Ž.; Jemec, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kahru, A. <i>et al.</i>","Englishabstract":"Brine shrimp <span><span><a href=\"/topics/earth-and-planetary-sciences/artemia\" title=\"Learn more about Artemia from ScienceDirect's AI-generated Topic Pages\" class=\"topic-link\">Artemia</a></span></span><span> sp. has been recognised as an important ecotoxicity and nanotoxicity test model organism for salt-rich aquatic environments, but currently there is still no harmonised testing protocol which would ensure the comparable results for hazard identification. In this paper we aimed to design the harmonised protocol for <a href=\"/topics/earth-and-planetary-sciences/nanomaterial\" title=\"Learn more about nanomaterial from ScienceDirect's AI-generated Topic Pages\" class=\"topic-link\">nanomaterial</a> toxicity testing using </span><em>Artemia franciscana</em><span> and present a case study to validate the protocol with silver <a href=\"/topics/earth-and-planetary-sciences/nanoparticle\" title=\"Learn more about nanoparticles from ScienceDirect's AI-generated Topic Pages\" class=\"topic-link\">nanoparticles</a> (AgNPs). We (i) revised the existing nanotoxicity test protocols with </span><em>Artemia</em><span> sp. (ii) optimised certain methodological steps based on the experiments with AgNPs and potassium <a href=\"/topics/earth-and-planetary-sciences/chromate\" title=\"Learn more about dichromate from ScienceDirect's AI-generated Topic Pages\" class=\"topic-link\">dichromate</a> (K</span><sub>2</sub>Cr<sub>2</sub>O<sub>7</sub>) as a soluble reference chemical and (iii) tested the optimised protocol in an international inter-laboratory exercise conducted within the EU FP7 NanoValid project. The intra- and inter-laboratory reproducibility of the proposed protocol with a soluble reference chemical K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub><span> was good, which confirms the suitability of this assay for conventional chemicals. However, the variability of AgNPs toxicity results was very high showing again that nanomaterials are inherently challenging for toxicity studies, especially those which toxic effect is linked to shed <a href=\"/topics/earth-and-planetary-sciences/metal-ion\" title=\"Learn more about metal ions from ScienceDirect's AI-generated Topic Pages\" class=\"topic-link\">metal ions</a>. Among the identified sources for this variability were: the hatching conditions, the type of test plate incubation and the illumination regime. The latter induced variations assumingly due to the changes in bioavailable silver species concentrations. Up to our knowledge this is the first inter-laboratory comparison of the </span><em>Artemia</em> sp. toxicity study involving nanomaterials. Although the inter-laboratory exercise revealed poor repeatability of AgNPs toxicity results, this study provides valuable information regarding the importance of harmonisation of all steps in the test procedure. Also, the presented AgNPs toxicity case study may serve as a platform for further validation steps with other types of NMs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A case study to optimise and validate the brine shrimp Artemia franciscana immobilisation assay with silver nanoparticles: The role of harmonisation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-23 01:33:22.681745","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2023-05-19","DateCreate":"2023-05-19","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000377921800020","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.envpol.2016.02.015"},"refs":null,"anarec":{"AnaID":364435,"PubliDate":2016,"Pagination":"173-183","XtraPublOfAnaID":null,"ISBN":null,"Volume":"213","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42834,"SerRR":"Environmental Pollution. 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