{"refrec":{"BRefID":364467,"RR":"<b>Melin, P.; Håkansson, S.; Schnürer, J.</b> (2006). Optimisation and comparison of liquid and dry formulations of the biocontrol yeast <i>Pichia anomala</i> J121. <i>Appl. Microbiol. Biotechnol. 73(5)</i>: 1008-1016. <a href=\"https://dx.doi.org/10.1007/s00253-006-0552-x\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-006-0552-x</a>","BEntID":362187,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Microbiol. Biotechnol. 73(5)</i>: 1008-1016. <a href=\"https://dx.doi.org/10.1007/s00253-006-0552-x\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-006-0552-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Melin, P.; Håkansson, S.; Schnürer, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Melin, P.; Håkansson, S.; Schnürer, J.","Englishabstract":"            The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations.    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