{"refrec":{"BRefID":365609,"RR":"<b>Sinninghe Damsté, J.S; Rijpstra, W.I.C.; Huber, K.J.; Albuquerque, L.; Egas, C.; Bale, N.</b> (2023). Dominance of mixed ether/ester, intact polar membrane lipids in five species of the order Rubrobacterales: Another group of bacteria not obeying the “lipid divide”. <i>Syst. Appl. Microbiol. 46(2)</i>: 126404. <a href=\"https://dx.doi.org/10.1016/j.syapm.2023.126404\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2023.126404</a>","BEntID":363331,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Syst. Appl. Microbiol. 46(2)</i>: 126404. <a href=\"https://dx.doi.org/10.1016/j.syapm.2023.126404\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2023.126404</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sinninghe Damsté, J.S; Rijpstra, W.I.C.; Huber, K.J.; Albuquerque, L.; Egas, C.; Bale, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sinninghe Damsté, J.S <i>et al.</i>","Englishabstract":"The composition of the core lipids and intact polar lipids (IPLs) of five Rubrobacter species was examined. Methylated (ω-4) fatty acids (FAs) characterized the core lipids of Rubrobacter radiotolerans, R. xylanophilus and R. bracarensis. In contrast, R. calidifluminis and R. naiadicus lacked ω-4 methyl FAs but instead contained abundant (i.e., 34–41 % of the core lipids) ω-cyclohexyl FAs not reported before in the order Rubrobacterales. Their genomes contained an almost complete operon encoding proteins enabling production of cyclohexane carboxylic acid CoA thioester, which acts as a building block for ω-cyclohexyl FAs in other bacteria. Hence, the most plausible explanation for the biosynthesis of these cyclic FAs in R. calidifluminis and R. naiadicus is a recent acquisition of this operon. All strains contained 1-O-alkyl glycerol ether lipids in abundance (up to 46 % of the core lipids), in line with the dominance (>90 %) of mixed ether/ester IPLs with a variety of polar headgroups. The IPL head group distribution of R. calidifluminis and R. naiadicus differed, e.g. they lacked a novel IPL tentatively assigned as phosphothreoninol. The genomes of all five Rubrobacter species contained a putative operon encoding the synthesis of the 1-O-alkyl glycerol phosphate, the presumed building block of mixed ether/ester IPLs, which shows some resemblance with an operon enabling ether lipid production in various other aerobic bacteria but requires more study. The uncommon dominance of mixed ether/ester IPLs in Rubrobacter species exemplifies our recent growing awareness that the lipid divide between archaea and bacteria/eukaryotes is not as clear cut as previously thought.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Dominance of mixed ether/ester, intact polar membrane lipids in five species of the order Rubrobacterales: Another group of bacteria not obeying the “lipid divide”","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:50.682984","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Rubrobacterales; 1-O-alkyl glycerol ether lipids; Intact polar lipids; ?-cyclohexyl fatty acids; (?-4)-methyl fatty acids; Rubrobacter radiotolerans; R. xylanophilus; R. bracarensis; R. calidifluminis; R. naiadicus","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-11-27","DateCreate":"2023-07-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000991959200001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.syapm.2023.126404"},"refs":null,"anarec":{"AnaID":365609,"PubliDate":2023,"Pagination":"126404","XtraPublOfAnaID":null,"ISBN":null,"Volume":"46","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43944,"SerRR":"Systematic and Applied Microbiology. 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