{"refrec":{"BRefID":365767,"RR":"<b>Schwartz-Narbonne, R.; Schaeffer, P.; Lengger, S.K.; Blewett, J.; Martin Jones, D.; Motsch, E.; Crombie, A.; Jetten, M.S.M.; Mikkelsen, D.; Normand, P.; Nuijten, G.H.L.; Pancost, R.D.; Rush, D.</b> (2023). Bacterial physiology highlighted by the δ13C fractionation of bacteriohopanetetrol isomers. <i>Org. Geochem. 181</i>: 104617. <a href=\"https://dx.doi.org/10.1016/j.orggeochem.2023.104617\" target=\"_blank\">https://dx.doi.org/10.1016/j.orggeochem.2023.104617</a>","BEntID":363489,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Org. Geochem. 181</i>: 104617. <a href=\"https://dx.doi.org/10.1016/j.orggeochem.2023.104617\" target=\"_blank\">https://dx.doi.org/10.1016/j.orggeochem.2023.104617</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Schwartz-Narbonne, R.; Schaeffer, P.; Lengger, S.K.; Blewett, J.; Martin Jones, D.; Motsch, E.; Crombie, A.; Jetten, M.S.M.; Mikkelsen, D.; Normand, P.; Nuijten, G.H.L.; Pancost, R.D.; Rush, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Schwartz-Narbonne, R. <i>et al.</i>","Englishabstract":"Lipid biomarkers, such as the various bacteriohopanetetrol  (BHT) isomers    studied here, are useful tools in tracing bacterially mediated  nitrogen and    carbon cycle processes affecting greenhouse gas emissions,  including the    anaerobic oxidation of ammonia. Three BHT isomers occur commonly  in the    environment. By gas chromatography, BHT-34 <i>S</i> elutes first; it  is    produced by numerous bacteria. The two later eluting isomers are more    constrained in their origin. The marine anammox bacteria ‘ <i>Ca.</i>    Scalindua’  is the only known producer of a BHT isomer of unknown    stereochemistry (BHT-<i>x</i>), making BHT- <i>x</i> a diagnostic biomarker    in anoxic marine settings. The  BHT-34 <i>R</i> isomer is produced by three    freshwater aerobic  heterotrophic producers ( <i>Frankia</i> spp.,    <i>        Acetobacter  pasteurianus    </i>    , and <i>Komagataeibacter xylinus</i>), a freshwater  serine-cycle (Type II)    methanotroph (<i>Methylocella palustris</i>), and the  freshwater anammox ‘    <i>Ca.</i> Brocadia’, which makes the detection of  freshwater anammox using    BHT-34 <i>R</i> more complicated. We investigated  whether the source of    BHT-34 <i>R</i> in freshwater environments could be  ascertained <i>via</i>    its δ<sup>13</sup>C value. We used  conventional on-column gas    chromatography-combustion-isotope ratio mass  spectrometry (GC-C-IRMS) (as    opposed to high temperature GC-C-IRMS) to  determine the <i>δ</i><sup>13</sup>C     composition of acetylated BHT isomers  in cultured bacteria and bacterial    enrichments. We combined these with bulk  biomass and substrate <i>δ</i><sup>13</sup>C     compositions to establish  carbon isotopic fractionation factors. The two    anammox genera had large  fractionation factors from dissolved inorganic    carbon (DIC) to biomass (Δ<sup>13</sup>C <sub>biomass  – DIC</sub> = –43.8    to –26.4 ‰) and to BHTs (Δ<sup>13</sup>C <sub>BHT  – DIC</sub> = –53.8 to    –38.2 ‰), which clearly distinguished them  from the freshwater aerobic    heterotrophic producers (Δ<sup>13</sup>C <sub>biomass  – substrate</sub> =    –2.3 to –0.1 ‰; Δ<sup>13</sup>C <sub>BHT –  substrate</sub> = –12.8 to 5.2    ‰). <i>Methylocella</i> assimilated  mainly carbon from DIC, rather than    from methane, into its biomass and BHT, and  previous work suggested this    assimilation comes with relatively small  fractionation. Thus, in peatlands,    the BHT δ<sup>13</sup>C values of <i>Methylocella</i> would  not reflect the    low δ<sup>13</sup>C values of biogenic methane. Consequently,  the presence    of BHT-34 <i>R</i> with low δ<sup>13</sup>C values relates to  ‘ <i>Ca.</i>    Brocadia’ and presents a novel tool to trace anammox in  freshwater    environments.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Bacterial physiology highlighted by the δ13C fractionation of bacteriohopanetetrol isomers","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-11 01:33:01.839261","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Carbon isotopes; Bacteriohopanepolyols; Anammox; Methanotroph; Bacteriohopanetetrols; Carbon-13; Lipid biomarkers; Hopanoids","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-07-20","DateCreate":"2023-07-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001001771100001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.orggeochem.2023.104617"},"refs":null,"anarec":{"AnaID":365767,"PubliDate":2023,"Pagination":"104617","XtraPublOfAnaID":null,"ISBN":null,"Volume":"181","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":111331,"SerRR":"Organic Geochemistry. Elsevier: Oxford; New York.  ISSN 0146-6380; e-ISSN 1873-5290","StandardTitleSer":"Organic Geochemistry","ISSN":"0146-6380","AbbrevSer":"Org. Geochem.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Schwartz-Narbonne","Firstname":"Rachel","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":400233,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schaeffer","Firstname":"Philippe","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":186306,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lengger","Firstname":"Sabine","Initials":"S.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":401389,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Blewett","Firstname":"Jerome","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":400232,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Martin Jones","Firstname":null,"Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":403481,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Motsch","Firstname":"Estelle","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":535457,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Crombie","Firstname":"Andrew","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":535458,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Jetten","Firstname":"Mike","Initials":"M.S.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":36808,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Mikkelsen","Firstname":"Deirdre","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":535460,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Normand","Firstname":"Philippe","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":403487,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Nuijten","Firstname":"Guylaine","Initials":"G.H.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":535462,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Pancost","Firstname":"Richard","Initials":"R.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":262761,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Rush","Firstname":"Darci","Initials":"D.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":363489,"AutID":252888,"OrderNr":13,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0001-8968-6042","PersID":29924,"InsID":13655}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":363489,"BEntPrivate":null,"availability":[{"BInstID":391192,"LibID":2779,"BRefID":365767,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"391192","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Elsevier","City":"Oxford; New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":363489,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":363489,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.orggeochem.2023.104617","externalID":"10.1016/j.orggeochem.2023.104617","URLTypeCode":"DOI","URLID":118066,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"daan.van.loon@nioz.nl","newSesDate":{"date":"2023-07-20 10:19:06.600000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"daan.van.loon@nioz.nl","updSesDate":{"date":"2023-07-20 10:19:06.600000","timezone_type":3,"timezone":"Europe/Brussels"}}}