{"refrec":{"BRefID":365886,"RR":"<b>Borges Monteiro, J.R.; Rodrigues, R.P.; Mazzuco, A.C.; de Cassia Ribeiro Gonçalves, R.; Bernardino, A.F.; Kuster, R.M.; Kitagawa, R.R.</b> (2023). In vitro and in silico evaluation of red algae <i>Laurencia obtusa</i> anticancer activity. <i>Mar. Drugs 21(6)</i>: 318. <a href=\"https://dx.doi.org/10.3390/md21060318\" target=\"_blank\">https://dx.doi.org/10.3390/md21060318</a>","BEntID":363608,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Drugs 21(6)</i>: 318. <a href=\"https://dx.doi.org/10.3390/md21060318\" target=\"_blank\">https://dx.doi.org/10.3390/md21060318</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Borges Monteiro, J.R.; Rodrigues, R.P.; Mazzuco, A.C.; de Cassia Ribeiro Gonçalves, R.; Bernardino, A.F.; Kuster, R.M.; Kitagawa, R.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Borges Monteiro, J.R. <i>et al.</i>","Englishabstract":"<div class=\"html-p\">Studies estimate that nearly 2 million new cases of gastric cancer will occur worldwide during the next two decades, which will increase mortality associated with cancer and the demand for new treatments. Marine algae of the <span class=\"html-italic\">Laurencia</span> genus have secondary metabolites known for their cytotoxic action, such as terpenes and acetogenins. The species <span class=\"html-italic\">Laurencia obtusa</span> has demonstrated cytotoxicity against many types of tumors in previous analyses. In this study, we determined the structure of terpenes, acetogenins, and one fatty acid of <span class=\"html-italic\">Laurencia</span> using mass spectrometry (ESI-FT-ICR/MS). In vitro cytotoxicity assays were performed with adenocarcinoma gastric cells (AGS) to select the most cytotoxic fraction of the crude extract of <span class=\"html-italic\">L. obtusa</span>. The Hex:AcOEt fraction was the most cytotoxic, with IC<sub>50</sub> 9.23 µg/mL. The selectivity index of 15.56 shows that the Hex:AcOEt fraction is selective to cancer cells. Compounds obtained from <span class=\"html-italic\">L. obtusa</span> were tested by the analysis of crystallographic complexes. Molecular docking calculations on the active site of the HIF-2α protein showed the highest affinity for sesquiterpene chermesiterpenoid B, identified from HEX:AcOEt fraction, reaching a score of 65.9. The results indicate that <span class=\"html-italic\">L. obtusa</span> presents potential compounds to be used in the treatment of neoplasms, such as gastric adenocarcinoma.</div>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"In vitro and in silico evaluation of red algae <i>Laurencia obtusa</i> anticancer activity","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-12 01:32:17.438516","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"gastric cancer; molecular docking","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-07-31","DateCreate":"2023-07-31","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001014881500001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/md21060318"},"refs":null,"anarec":{"AnaID":365886,"PubliDate":2023,"Pagination":"318","XtraPublOfAnaID":null,"ISBN":null,"Volume":"21","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":113252,"SerRR":"Marine Drugs. 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