{"refrec":{"BRefID":366331,"RR":"<b>Senn, S.; Bhattacharyya, S.; Presley, G.; Taylor, A.E.; Stanis, R.; Pangell, K.; Melendez, D.; Ford, J.</b> (2023). The community structure of eDNA in the Los Angeles River reveals an altered nitrogen cycle at impervious sites. <i>Diversity 15(7)</i>: 823. <a href=\"https://dx.doi.org/10.3390/d15070823\" target=\"_blank\">https://dx.doi.org/10.3390/d15070823</a>","BEntID":364053,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Diversity 15(7)</i>: 823. <a href=\"https://dx.doi.org/10.3390/d15070823\" target=\"_blank\">https://dx.doi.org/10.3390/d15070823</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Senn, S.; Bhattacharyya, S.; Presley, G.; Taylor, A.E.; Stanis, R.; Pangell, K.; Melendez, D.; Ford, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Senn, S. <i>et al.</i>","Englishabstract":"In this study, we sought to investigate the impact of urbanization, the presence of concrete river bottoms, and nutrient pollution on microbial communities along the L.A. River. Six molecular markers were evaluated for the identification of bacteria, plants, fungi, fish, and invertebrates in 90 samples. PCA (principal components analysis) was used along with PAM (partitioning around medoids) clustering to reveal community structure, and an NB (negative binomial) model in DESeq2 was used for differential abundance analysis. PCA and factor analysis exposed the main axes of variation but were sensitive to outliers. The differential abundance of Proteobacteria was associated with soft-bottom sites, and there was an apparent balance in the abundance of bacteria responsible for nitrogen cycling. Nitrogen cycling was explained via ammonia-oxidizing archaea; the complete ammonia oxidizers, <i>Nitrospira</i> sp.; nitrate-reducing bacteria, <i>Marmoricola</i> sp.; and nitrogen-fixing bacteria <i>Devosia</i> sp., which were differentially abundant at soft-bottom sites (p adj < 0.002). In contrast, the differential abundance of several <i>cyanobacteria</i> and other anoxygenic phototrophs was associated with the impervious sites, which suggested the accumulation of excess nitrogen. The soft-bottom sites tended to be represented by a differential abundance of aerobes, whereas the concrete-associated species tended to be alkaliphilic, saliniphilic, calciphilic, sulfate dependent, and anaerobic. In the Glendale Narrows, downstream from multiple water reclamation plants, there was a differential abundance of cyanobacteria and algae; however, indicator species for low nutrient environments and ammonia-abundance were also present. There was a differential abundance of ascomycetes associated with Arroyo Seco and a differential abundance of <i>Scenedesmaceae</i> green algae and cyanobacteria in Maywood, as seen in the analysis that compared suburban with urban river communities. The proportion of Ascomycota to Basidiomycota within the L.A. River differed from the expected proportion based on published worldwide freshwater and river 18S data; the shift in community structure was most likely associated with the extremes of urbanization. This study indicates that extreme urbanization can result in the overrepresentation of cyanobacterial species that could cause reductions in water quality and safety.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The community structure of eDNA in the Los Angeles River reveals an altered nitrogen cycle at impervious sites","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"metabarcoding; microbial communities; nitrogen cycling; statistical modeling; water quality; urban river ecology","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-08-28","DateCreate":"2023-08-28","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001035067900001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/d15070823"},"refs":null,"anarec":{"AnaID":366331,"PubliDate":2023,"Pagination":"823","XtraPublOfAnaID":null,"ISBN":null,"Volume":"15","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":239468,"SerRR":"Diversity. 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