{"refrec":{"BRefID":366422,"RR":"<b>Lund, P.K.</b> (2023). Host-microbe interactions in non-native estuarine anemones: biogeography and temperature. MSc Thesis. Faculty of California State Polytechnic University: Humboldt. xv, 143 pp.","BEntID":364146,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". MSc Thesis. Faculty of California State Polytechnic University: Humboldt.  xv, 143 pp.","DocTypID":5,"DocType":"Book/Monograph","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lund, P.K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lund, P.K.","Englishabstract":"Non-native species are increasing in prevalence around the world, resulting in negative economic and ecological impacts. However, the broad distributions of non-native species also offer a system for investigating the response of host-associated microbial communities to environmental factors across a range of ecological scales. At the broadest scale, I investigated the geography of microbial communities in the non-native estuarine anemone <em>Diadumene lineata</em> on the west coast of the United States of America. Across latitudes, microbial community composition was very similar and displayed a high percentage of <em>Klebsiella spp. </em>at all sites. However, the communities in California tended to exhibit higher richness, Shannon-Wiener diversity, and beta-dispersion than the communities in Oregon and Washington, driven by an abundance of <em>Desulfobacterota</em>. In a stress experiment, where three anemone species (<em>Diadumene lineata</em>, <em>Diadumene leucolena</em>, and <em>Metridium senile</em>) were subjected to a gradient from 0-40°C to evaluate each species’ capacity for buffering their microbial community against thermal stress, I found species-specific patterns across temperatures, with only <em>M. senile </em>exhibiting evidence of buffering at moderate temperatures. In contrast, <em>D. lineata</em> and <em>D. leucolena </em>did not appear to be buffering their microbial communities, with <em>D. lineata</em> displaying unique community compositions across temperatures, while the communities on <em>D. leucolena</em> generally exhibited high beta-dispersion. Finally, I isolated anemone-associated bacteria on a novel medium made of anemone tissue and measured their growth from 30-40°C, identifying candidates for beneficial host-microbe interactions in warm environments. The anemone-based medium overwhelmingly selected for the genera <em>Pseudoalteromonas</em> and <em>Peribacillis,</em> regardless of anemone host species. <em>Peribacillus spp.</em> were particularly thermal tolerant, growing similarly from 30-40°C, while <em>Pseudoalteromonas spp.</em> grew well from 30-35°C. The remaining tested genera preferred 30°C, however one of the <em>Litoreibacter sp.</em> produced a putative melanin that may protect cells against thermal stress. This is the first study exploring microbial communities in the non-native estuarine anemone <em>D. lineata</em> and lays the foundation for an expanded global assessment of latitudinal gradients, investigating how additional abiotic factors like genotype and pH drive microbial community composition, and directly testing beneficial host-microbe interactions with isolated bacteria.","AbstractOtherLang":null,"BibLvlCode":"M","StandardTitle":"Host-microbe interactions in non-native estuarine anemones: biogeography and temperature","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":2023,"DateUpdate":"2023-09-04","DateCreate":"2023-09-04","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":null,"monrec":{"MonID":366422,"ISBN":null,"PubliDate":2023,"IssueDate":null,"Volume":null,"Issue":null,"Pagination":"xv, 143","Place":"Humboldt","Edition":null,"BRefXtra":null,"BRefXtraRR":null,"SerID":null,"SerRR":null,"Ser2BRefID":null,"Ser2RR":null,"StandardTitleSer":null,"ISSN":null,"AbbrevSer":null,"Degree":"MSc","ThesisID":366422,"InsID":null,"Acronym":null,"FullStandardName":null,"ToPubliDate":null,"SerNotes":null,"eISBN":null,"Pages":143},"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lund","Firstname":"Parker","Initials":"P.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":364146,"AutID":537642,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":391932,"LibID":36,"BRefID":366422,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"391932","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":[{"LitStyID":7,"Style":"Dissertation"}],"thespers":null,"arch2discl":null,"SERpubls":null,"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"}},"doi":null,"publs":[{"PublID":33335,"PublName":"Faculty of California State Polytechnic University","InsID":null,"PersID":null,"INBOID":null,"OrderNr":1}],"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":364146,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":364146,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2023-09-04 08:33:28.530000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2023-09-04 08:33:28.530000","timezone_type":3,"timezone":"Europe/Brussels"}}}