{"refrec":{"BRefID":366792,"RR":"<b>Seelam, J.S.; Fernandes de Souza, M.; Chaerle, P.; Willems, B.; Michels, E.; Vyverman, W.; Meers, E.</b> (2022). Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application. <i>Chemosphere 290</i>: 133180. <a href=\"https://dx.doi.org/10.1016/j.chemosphere.2021.133180\" target=\"_blank\">https://dx.doi.org/10.1016/j.chemosphere.2021.133180</a>","BEntID":364516,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Chemosphere 290</i>: 133180. <a href=\"https://dx.doi.org/10.1016/j.chemosphere.2021.133180\" target=\"_blank\">https://dx.doi.org/10.1016/j.chemosphere.2021.133180</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Seelam, J.S.; Fernandes de Souza, M.; Chaerle, P.; Willems, B.; Michels, E.; Vyverman, W.; Meers, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Seelam, J.S. <i>et al.</i>","Englishabstract":"The integration of phototrophic microalgal production and anaerobic digestion can recycle excess nutrients across European surplus hotspots to produce protein-rich biomass for nutritional applications. However, the challenging physico-chemical properties of raw digestate constrain microalgal growth and limit digestate valorization potential. This study focused on the pre-treatment of food waste-based digestate using paper-filtration to improve its properties for cultivating Desmodesmus sp. and Chlorella vulgaris. The microalgal growth performance in paper-filtered digestate (PFD, 10 μm-pore size) was then compared to growth in membrane-filtered digestate (MFD, 0.2 μm-pore size). A microplate-based screening coupled with Cytation device assessment of PFD and MFD samples after dilution and with/without phosphorus supplementation showed that PFD was the best substrate. Moreover, phosphorus supplementation resulted in improved growth at higher digestate concentrations (5–10% v/v PFD), indicating the importance of using a balanced growth medium to increase the volumetric usage of digestate. Results were validated in a 3-L bioreactor at 10% PFD with phosphorus supplementation, reaching a biomass concentration of 2.4 g L−1 with a protein and carbohydrate content of 67% and 13% w/w respectively. This trial indicates that paper-filtration is a promising pre-treatment technique to maximize digestate recycling and deliver a sustainable animal feed-grade protein alternative.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Digestate; Microalgae; Microplate experiments; Paper-filtration; Photobioreactor","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-09-18","DateCreate":"2023-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000734096600005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.chemosphere.2021.133180"},"refs":null,"anarec":{"AnaID":366792,"PubliDate":2022,"Pagination":"133180","XtraPublOfAnaID":null,"ISBN":null,"Volume":"290","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42564,"SerRR":"Chemosphere. 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