{"refrec":{"BRefID":367049,"RR":"<b>Le Loarer, A.; Marcellin-Gros, R.; Dufossé, L.; Bignon, J.; Frédérich, M.; Ledoux, A.; Queiroz, E.F.; Wolfender, J.-L.; Gauvin-Bialecki, A.; Fouillaud, M.</b> (2023). Prioritization of microorganisms isolated from the Indian Ocean sponge <i>Scopalina hapalia</i> based on metabolomic diversity and biological activity for the discovery of natural products. <i>Microorganisms 11(3)</i>: 697. <a href=\"https://dx.doi.org/10.3390/microorganisms11030697\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms11030697</a>","BEntID":364773,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Microorganisms 11(3)</i>: 697. <a href=\"https://dx.doi.org/10.3390/microorganisms11030697\" target=\"_blank\">https://dx.doi.org/10.3390/microorganisms11030697</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Le Loarer, A.; Marcellin-Gros, R.; Dufossé, L.; Bignon, J.; Frédérich, M.; Ledoux, A.; Queiroz, E.F.; Wolfender, J.-L.; Gauvin-Bialecki, A.; Fouillaud, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Le Loarer, A. <i>et al.</i>","Englishabstract":"<div class=\"html-p\">Despite considerable advances in medicine and technology, humanity still faces many deadly diseases such as cancer and malaria. In order to find appropriate treatments, the discovery of new bioactive substances is essential. Therefore, research is now turning to less frequently explored habitats with exceptional biodiversity such as the marine environment. Many studies have demonstrated the therapeutic potential of bioactive compounds from marine macro- and microorganisms. In this study, nine microbial strains isolated from an Indian Ocean sponge, <span class=\"html-italic\">Scopalina hapalia,</span> were screened for their chemical potential. The isolates belong to different phyla, some of which are already known for their production of secondary metabolites, such as the actinobacteria. This article aims at describing the selection method used to identify the most promising microorganisms in the field of active metabolites production. The method is based on the combination of their biological and chemical screening, coupled with the use of bioinformatic tools. The dereplication of microbial extracts and the creation of a molecular network revealed the presence of known bioactive molecules such as staurosporin, erythromycin and chaetoglobosins. Molecular network exploration indicated the possible presence of novel compounds in clusters of interest. The biological activities targeted in the study were cytotoxicity against the HCT-116 and MDA-MB-231 cell lines and antiplasmodial activity against <span class=\"html-italic\">Plasmodium falciparum</span> 3D7. <span class=\"html-italic\">Chaetomium globosum</span> SH-123 and <span class=\"html-italic\">Salinispora arenicola</span> SH-78 strains actually showed remarkable cytotoxic and antiplasmodial activities, while <span class=\"html-italic\">Micromonospora fluostatini</span> SH-82 demonstrated promising antiplasmodial effects. The ranking of the microorganisms as a result of the different screening steps allowed the selection of a promising strain, <span class=\"html-italic\">Micromonospora fluostatini</span> SH-82, as a premium candidate for the discovery of new drugs.</div>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Prioritization of microorganisms isolated from the Indian Ocean sponge <i>Scopalina hapalia</i> based on metabolomic diversity and biological activity for the discovery of natural products","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-09-25 01:36:50.712317","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"natural products; marine microorganisms; specialized metabolites; molecular network; dereplication; cytotoxic activity; antiplasmodial activity; prioritization method","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2023-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000958744400001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/microorganisms11030697"},"refs":null,"anarec":{"AnaID":367049,"PubliDate":2023,"Pagination":"697","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":302866,"SerRR":"Microorganisms. 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