{"refrec":{"BRefID":367068,"RR":"<b>Tilbury, M.A.; Tran, T.Q.; Shingare, D.; Lefevre, M.; Power, A.M.; Leclère, P.; Wall, J.G.</b> (2023). Self-assembly of a barnacle cement protein into intertwined amyloid fibres and determination of their adhesive and viscoelastic properties. <i>J. R. Soc. Interface 20(205)</i>: 20230332. <a href=\"https://dx.doi.org/10.1098/rsif.2023.0332\" target=\"_blank\">https://dx.doi.org/10.1098/rsif.2023.0332</a>","BEntID":364792,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. R. Soc. Interface 20(205)</i>: 20230332. <a href=\"https://dx.doi.org/10.1098/rsif.2023.0332\" target=\"_blank\">https://dx.doi.org/10.1098/rsif.2023.0332</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tilbury, M.A.; Tran, T.Q.; Shingare, D.; Lefevre, M.; Power, A.M.; Leclère, P.; Wall, J.G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tilbury, M.A. <i>et al.</i>","Englishabstract":"The stalked barnacle Pollicipes pollicipes uses a multi-protein cement to adhere to highly varied substrates in marine environments. We investigated the morphology and adhesiveness of a component 19 kDa protein in barnacle cement gland- and seawater-like conditions, using transmission electron microscopy and state-of-the art scanning probe techniques. The protein formed amyloid fibres after 5 days in gland-like but not seawater conditions. After 7–11 days, the fibres self-assembled under gland-like conditions into large intertwined fibrils of up to 10 µm in length and 200 nm in height, with a distinctive twisting of fibrils evident after 11 days. Atomic force microscopy (AFM)-nanodynamic mechanical analysis of the protein in wet conditions determined E′ (elasticity), E′′ (viscosity) and tan δ values of 2.8 MPa, 1.2 MPa and 0.37, respectively, indicating that the protein is a soft and viscoelastic material, while the adhesiveness of the unassembled protein and assembled fibres, measured using peak force quantitative nanomechanical mapping, was comparable to that of the commercial adhesive Cell-Tak™. The study provides a comprehensive insight into the nanomechanical and viscoelastic properties of the barnacle cement protein and its self-assembled fibres under native-like conditions and may have application in the design of amyloid fibril-based biomaterials or bioadhesives.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Self-assembly of a barnacle cement protein into intertwined amyloid fibres and determination of their adhesive and viscoelastic properties","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:32:22.143309","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"protein adhesion;barnacle cement protein;viscoelastic nanoscale properties;amyloid fibre;self-assembly;FIBRIL FORMATION;LEPAS-ANATIFERA","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2023-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001044358900006","VABBcode":null,"OpenAcc":1,"DOI":"10.1098/rsif.2023.0332"},"refs":null,"anarec":{"AnaID":367068,"PubliDate":2023,"Pagination":"20230332","XtraPublOfAnaID":null,"ISBN":null,"Volume":"20","Issue":"205","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":143378,"SerRR":"Journal of the Royal Society. 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