{"refrec":{"BRefID":367329,"RR":"<b>Leite, B.R.; Duarte, S.; Troncoso, J.S.; Costa, F.O.</b> (2023). Artificial seaweed substrates complement ARMS in DNA metabarcoding-based monitoring of temperate coastal macrozoobenthos. <i>Diversity 15(5)</i>: 657. <a href=\"https://dx.doi.org/10.3390/d15050657\" target=\"_blank\">https://dx.doi.org/10.3390/d15050657</a>","BEntID":365054,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Diversity 15(5)</i>: 657. <a href=\"https://dx.doi.org/10.3390/d15050657\" target=\"_blank\">https://dx.doi.org/10.3390/d15050657</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Leite, B.R.; Duarte, S.; Troncoso, J.S.; Costa, F.O.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Leite, B.R. <i>et al.</i>","Englishabstract":"We used DNA metabarcoding to compare macrozoobenthic species colonization between autonomous reef monitoring structures (ARMS) and artificial seaweed monitoring systems (ASMS). We deployed both substrates in two different locations (Ría de Vigo and Ría de Ferrol, NW Iberian coast) and collected them after 6, 9, and 12 months to assess species composition of the colonizing communities through high-throughput sequencing of amplicons within the barcode region of the mitochondrial cytochrome c oxidase I (COI-5P) and the V4 domain of the 18S rRNA genes. We observed a consistently low similarity in species composition between substrate types, independently of sampling times and sites. A large fraction of exclusive species was recorded for a given substrate (up to 72%), whereas only up to 32% of species were recorded in both substrates. The shape and structural complexity of the substrate strongly affected the colonization preferences, with ASMS detecting more exclusive crustacean and gastropod species and a broader diversity of taxonomic groups (e.g., Entoprocta and Pycnogonida were detected exclusively in ASMS). We demonstrate that despite the customary use of ARMS for macrozoobenthos monitoring, by using ASMS we complemented the recovery of species and enlarged the scope of the taxonomic diversity recorded.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Artificial seaweed substrates complement ARMS in DNA metabarcoding-based monitoring of temperate coastal macrozoobenthos","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"substrate type; artificial reef monitoring structures (ARMS); artificial seaweed monitoring system (ASMS); environmental DNA; COI; 18S","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-09-25","DateCreate":"2023-09-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000997856100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/d15050657"},"refs":null,"anarec":{"AnaID":367329,"PubliDate":2023,"Pagination":"657","XtraPublOfAnaID":null,"ISBN":null,"Volume":"15","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":239468,"SerRR":"Diversity. 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