{"refrec":{"BRefID":367478,"RR":"<b>Ramanjooloo, A.; Kamel, M.; Adeyemi, S.A.; Ubanako, P.; Baudot, B.; Thakoor, A.D.; Choonara, Y.E.; Bhaw-Luximon, A.</b> (2023). LC-MS/MS identification and cytotoxic assessment of jaspamide and its congeners from the sponge <i>Jaspis diastra</i>. <i>New Journal of Chemistry 47(18)</i>: 8854-8866. <a href=\"https://dx.doi.org/10.1039/d3nj00459g\" target=\"_blank\">https://dx.doi.org/10.1039/d3nj00459g</a>","BEntID":365203,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>New Journal of Chemistry 47(18)</i>: 8854-8866. <a href=\"https://dx.doi.org/10.1039/d3nj00459g\" target=\"_blank\">https://dx.doi.org/10.1039/d3nj00459g</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ramanjooloo, A.; Kamel, M.; Adeyemi, S.A.; Ubanako, P.; Baudot, B.; Thakoor, A.D.; Choonara, Y.E.; Bhaw-Luximon, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ramanjooloo, A. <i>et al.</i>","Englishabstract":"Jaspamide (jasplakinolide; NSC-613009) is a cytotoxic cyclic polypeptide that was initially isolated from the sponge <em>Jaspis cf. Johnston</em>. It displayed anti-tumor, anti-fungal and insecticidal activities, among others. Herein, an LC-MS/MS approach for the identification of jaspamide (<strong>1</strong>) and its congeners in the extracts of the sponge <em>Jaspis diastra</em>, collected from the Mauritius waters, is described. Based on the interpretation of LC-MS/MS data and using the proposed fragmentation pattern of jaspamide (<strong>1</strong>) as a model, jaspamide (<strong>1</strong>) and its congeners were identified in the extracts. Jaspamide (<strong>1</strong>), Q, R and Z<small><sub>1</sub></small> were identified in hexane and ethyl acetate extracts whereas jaspamide (<strong>1</strong>) and Z<small><sub>1</sub></small> were identified in the methanol extract. The cytotoxic evaluation of the extracts revealed that the ethyl acetate extract was more active than the hexane and methanol extracts with IC<small><sub>50</sub></small> values of 1.60 ± 0.48 μg mL<small><sup>−1</sup></small> on A172, 1.71 ± 0.42 μg mL<small><sup>−1</sup></small> on TERA1, 1.05 ± 0.02 μg mL<small><sup>−1</sup></small> on DLD1 and 0.75 ± 0.04 μg mL<small><sup>−1</sup></small> on HaCaT. Subsequent fractionation of this extract by column chromatography resulted in the isolation of a precipitate (JC-1) which was characterized by 1D and 2D NMR as jaspamide (<strong>1</strong>). Semi-preparative HPLC of JC-1 resulted in a purified jaspamide (<strong>1</strong>) (JC-1P). The purity of JC-1P was determined by analytical HPLC using commercial jaspamide with a purity of ≥97% as the reference. JC-1P with a purity of 87.63 ± 1.33% displayed IC<small><sub>50</sub></small> values of 1.30 ± 0.05 μg mL<small><sup>−1</sup></small> on A172, 2.59 ± 0.33 μg mL<small><sup>−1</sup></small> on TERA1, 0.68 ± 0.11 μg mL<small><sup>−1</sup></small> on DLD1 and 0.82 ± 0.19 μg mL<small><sup>−1</sup></small> on HaCaT.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"LC-MS/MS identification and cytotoxic assessment of jaspamide and its congeners from the sponge <i>Jaspis diastra</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-09-25","DateCreate":"2023-09-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000975905800001","VABBcode":null,"OpenAcc":0,"DOI":"10.1039/d3nj00459g"},"refs":null,"anarec":{"AnaID":367478,"PubliDate":2023,"Pagination":"8854-8866","XtraPublOfAnaID":null,"ISBN":null,"Volume":"47","Issue":"18","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":275384,"SerRR":"New Journal of Chemistry. ROYAL SOC CHEMISTRY: Cambridge.  ISSN 1144-0546; e-ISSN 1369-9261","StandardTitleSer":"New Journal of Chemistry","ISSN":"1144-0546","AbbrevSer":null,"StandardTitleMon":null,"StartPage":8854,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ramanjooloo","Firstname":"Avin","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541345,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kamel","Firstname":"Mahmoud","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541346,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Adeyemi","Firstname":"Samson","Initials":"S.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541347,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ubanako","Firstname":"Philemon","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541348,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Baudot","Firstname":"Bertrand","Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541349,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Thakoor","Firstname":"Asho","Initials":"A.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541350,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Choonara","Firstname":"Yahya","Initials":"Y.E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541351,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bhaw-Luximon","Firstname":"Archana","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":365203,"AutID":541352,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":392789,"LibID":36,"BRefID":367478,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"392789","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"ROYAL SOC CHEMISTRY","City":"Cambridge"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Jaspis diastra"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":365203,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":365203,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1039/d3nj00459g","externalID":"10.1039/d3nj00459g","URLTypeCode":"DOI","URLID":120073,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Jaspis diastra","AphiaID":233139,"TaxtID":43157}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Jaspis diastra","DutchTerm":null,"AphiaID":233139,"TaxtID":43157}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2023-09-25 08:17:28.120000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2023-09-25 08:17:28.120000","timezone_type":3,"timezone":"Europe/Brussels"}}}